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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61422dCAS9-VP64_GFPExpresses dCAS9-VP64 activator with 2A GFP
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61423MS2-P65-HSF1_GFPExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.
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61424sgRNA(MS2) cloning backbonesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
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61425lenti dCAS-VP64_Blast3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
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61426lenti MS2-P65-HSF1_Hygrolenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
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61427lenti sgRNA(MS2)_zeo backbone3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
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73795lenti sgRNA(MS2)_puro backbone3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
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