PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61423||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human), Synthetic
MutationN55K in MS2
- Promoter EF1A
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G
- 3′ sequencing primer cacatagcgtaaaaggagcaacatag (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
For additional information, protocols and an activator sgRNA design tool, visit our website:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MS2-P65-HSF1_GFP was a gift from Feng Zhang (Addgene plasmid # 61423)
For your References section:Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.