Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PubMed Article
Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72793 | pMK277 (mClover-NeoR) | C-terminal tagging with mClover using CRIPSR/Cas9 |
| 72794 | pMK278 (mClover-Hygro) | C-terminal tagging with mClover using CRIPSR/Cas9 |
| 72795 | pMK279 (mClover-Bsr) | C-terminal tagging with mClover using CRIPSR/Cas9 |
| 72796 | pMK280 (mCherry2-NeoR) | C-terminal tagging with mCherry2 using CRIPSR/Cas9 |
| 72797 | pMK281 (mCherry2-Hygro) | C-terminal tagging with mCherry2 using CRIPSR/Cas9 |
| 72798 | pMK282 (mCherry2-Bsr) | C-terminal tagging with mCherry2 using CRIPSR/Cas9 |
| 72799 | pMK283 (Stag-3FLAG-Neo) | C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9 |
| 72800 | pMK284 (Stag-3FLAG-Hygro) | C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9 |
| 72801 | pMK285 (Stag-3FLAG-Bsr) | C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9 |
| 72824 | pMK286 (mAID-NeoR) | C-terminal tagging with mAID using CRISPR/Cas9 |
| 72825 | pMK287 (mAID-Hygro) | C-terminal tagging with mAID using CRISPR/Cas9 |
| 72826 | pMK288 (mAID-Bsr) | C-terminal tagging with mAID using CRISPR/Cas9 |
| 72827 | pMK289 (mAID-mClover-NeoR) | C-terminal tagging with mAID-mClover using CRISPR/Cas9 |
| 72828 | pMK290 (mAID-mClover-Hygro) | C-terminal tagging with mAID-mClover using CRISPR/Cas9 |
| 72829 | pMK291 (mAID-mClover-Bsr) | C-terminal tagging with mAID-mClover using CRISPR/Cas9 |
| 72830 | pMK292 (mAID-mCherry2-NeoR) | C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9 |
| 72831 | pMK293 (mAID-mCherry2-Hygro) | C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9 |
| 72832 | pMK294 (mAID-mCherry2-Bsr) | C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9 |
| 72833 | AAVS1 T2 CRIPR in pX330 | CRISPR/Cas to target the AAVS1 locus in human cells |
| 72834 | pMK232 (CMV-OsTIR1-PURO) | Expresses OsTIR1 under the control of the CMV promoter |
| 72835 | pMK243 (Tet-OsTIR1-PURO) | Expresses OsTIR1 under the control of the Tet promoter |