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pMK287 (mAID-Hygro)
(Plasmid #72825)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72825 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS(-)
  • Backbone size w/o insert (bp) 2875
  • Total vector size (bp) 5663
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mAID-Hygro
  • Species
    H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken)
  • Insert Size (bp)
    2788
  • Tag / Fusion Protein
    • mAID (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GGAAACAGCTATGACCATG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This vector can be used for the construction of donor vectors for tagging with mAID.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK287 (mAID-Hygro) was a gift from Masato Kanemaki (Addgene plasmid # 72825)
  • For your References section:

    Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. 10.1016/j.celrep.2016.03.001 PubMed 27052166