PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72798||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS(-)
- Backbone size w/o insert (bp) 2871
- Total vector size (bp) 5557
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken)
/ Fusion Protein
- mCherry2 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GTAAAACGACGGCCAGT
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bymCherry2 is a kind gift from Dr. Michael Davidson (Addgene #54517).
Terms and Licenses
- Not Available to Industry
This vector can be used for the construction of donor vectors for tagging with mCherry2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMK282 (mCherry2-Bsr) was a gift from Masato Kanemaki (Addgene plasmid # 72798 ; http://n2t.net/addgene:72798 ; RRID:Addgene_72798)
For your References section:Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. 10.1016/j.celrep.2016.03.001 PubMed 27052166