169,069 results

1. pAK005

   Plasmid

   #233642

   Purpose

   For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   eSpCas9(1.1) with c-terminal VitelloTagB
   

   Tags

   6-His

   Expression

   Mammalian

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTagBFP2-Lifeact-7

   Plasmid

   #54602

   Purpose

   Localization: Actin, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Lifeact-mTagBFP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #162378-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pDL278_P23-DsRed-Express2

   Plasmid

   #121505

   Purpose

   E. coli-streptococcal shuttle vector that expresses rfp

   Depositor

   Robert Shields

   Article

   Shields et al Appl Environ Microbiol. 2019 May 17. pii

   Insert

   DsRed-Express2
   

   Use

   E. coli streptococcal shuttle vector

   Promoter

   P23

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNC02

   Plasmid

   #233644

   Purpose

   For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   sfGFP with c-terminal VitelloTagB
   

   Tags

   6-His

   Expression

   Bacterial

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-M-Flag-BAP1-C91A

   Plasmid

   #125841

   Purpose

   express mutant BAP1-C91A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   C91A

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-2xFLAG-SREBP-1c

   Plasmid

   #26802

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1c (SREBF1 Human)
   

   Tags

   2XFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_NOPlight1

   Plasmid

   #195578

   Purpose

   Expresses NOPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

   Insert

   NOPLight1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-Climp63

   Plasmid

   #136293

   Purpose

   expression of Climp63

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Cytoskeletal-linking protein 63 (Ckap4 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPaint Gene Tagging Kit

    Plasmid Kit

    #1000000086

    Purpose

    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

    Depositor

    Veit Hornung

    Available Since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

13. dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

    Viral Prep

    #61422-LVC

    Purpose

    Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Promoter

    EF1a

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pT18mobsacB

    Plasmid

    #72648

    Purpose

    sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Available Since

    Feb. 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3 CDK7 HA (P#633)

    Plasmid

    #14647

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuide-Hygro-iRFP670

    Plasmid

    #99377

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJR98

    Plasmid

    #187239

    Purpose

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH2B-emiRFP670

    Plasmid

    #136571

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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