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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro Citations (24)

Plasmid Article: Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
PubMed Journal

Articles Citing pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Articles
Dissecting super-enhancer hierarchy based on chromatin interactions. Huang J, Li K, Cai W, Liu X, Zhang Y, Orkin SH, Xu J, Yuan GC. Nat Commun. 2018 Mar 5;9(1):943. doi: 10.1038/s41467-018-03279-9. PubMed
High-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing. Zhang P, Xia JH, Zhu J, Gao P, Tian YJ, Du M, Guo YC, Suleman S, Zhang Q, Kohli M, Tillmans LS, Thibodeau SN, French AJ, Cerhan JR, Wang LD, Wei GH, Wang L. Nat Commun. 2018 May 22;9(1):2022. doi: 10.1038/s41467-018-04451-x. PubMed
A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans. Tsai PF, Dell'Orso S, Rodriguez J, Vivanco KO, Ko KD, Jiang K, Juan AH, Sarshad AA, Vian L, Tran M, Wangsa D, Wang AH, Perovanovic J, Anastasakis D, Ralston E, Ried T, Sun HW, Hafner M, Larson DR, Sartorelli V. Mol Cell. 2018 Jul 5;71(1):129-141.e8. doi: 10.1016/j.molcel.2018.06.008. PubMed
Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System. Moses C, Nugent F, Waryah CB, Garcia-Bloj B, Harvey AR, Blancafort P. Mol Ther Nucleic Acids. 2019 Mar 1;14:287-300. doi: 10.1016/j.omtn.2018.12.003. Epub 2018 Dec 14. PubMed
The Crohn's disease associated SNP rs6651252 impacts MYC gene expression in human colonic epithelial cells. Matthews SM, Eshelman MA, Berg AS, Koltun WA, Yochum GS. PLoS One. 2019 Feb 22;14(2):e0212850. doi: 10.1371/journal.pone.0212850. eCollection 2019. PubMed
Applications of Lgr5-Positive Cochlear Progenitors (LCPs) to the Study of Hair Cell Differentiation. Lenz DR, Gunewardene N, Abdul-Aziz DE, Wang Q, Gibson TM, Edge ASB. Front Cell Dev Biol. 2019 Feb 19;7:14. doi: 10.3389/fcell.2019.00014. eCollection 2019. PubMed
Increased Serine Synthesis Provides an Advantage for Tumors Arising in Tissues Where Serine Levels Are Limiting. Sullivan MR, Mattaini KR, Dennstedt EA, Nguyen AA, Sivanand S, Reilly MF, Meeth K, Muir A, Darnell AM, Bosenberg MW, Lewis CA, Vander Heiden MG. Cell Metab. 2019 Jun 4;29(6):1410-1421.e4. doi: 10.1016/j.cmet.2019.02.015. Epub 2019 Mar 21. PubMed
Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs. Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, Theunissen TW, Jaenisch R, Trono D. Cell Stem Cell. 2019 May 2;24(5):724-735.e5. doi: 10.1016/j.stem.2019.03.012. Epub 2019 Apr 18. PubMed
The enhancer RNA ARIEL activates the oncogenic transcriptional program in T-cell acute lymphoblastic leukemia. Tan SH, Leong WZ, Ngoc PCT, Tan TK, Bertulfo FC, Lim MC, An O, Li Z, Yeoh AEJ, Fullwood MJ, Tenen DG, Sanda T. Blood. 2019 Jul 18;134(3):239-251. doi: 10.1182/blood.2018874503. Epub 2019 May 10. PubMed
Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cells. Delfarah A, Parrish S, Junge JA, Yang J, Seo F, Li S, Mac J, Wang P, Fraser SE, Graham NA. J Biol Chem. 2019 Jul 5;294(27):10564-10578. doi: 10.1074/jbc.RA118.005806. Epub 2019 May 28. PubMed
An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis. Mayank AK, Pandey V, Vashisht AA, Barshop WD, Rayatpisheh S, Sharma T, Haque T, Powers DN, Wohlschlegel JA. Mol Cell. 2019 Jul 25;75(2):382-393.e5. doi: 10.1016/j.molcel.2019.05.020. Epub 2019 Jun 19. PubMed
CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression. Weng J, Han X, Liu K, Yang J, Wei S, Zhang Y, Zeng F, Li Y, Shen L, Gao Y. Int J Biol Sci. 2019 Jun 4;15(8):1664-1675. doi: 10.7150/ijbs.35216. eCollection 2019. PubMed
Epigenome editing strategies for the functional annotation of CTCF insulators. Tarjan DR, Flavahan WA, Bernstein BE. Nat Commun. 2019 Sep 18;10(1):4258. doi: 10.1038/s41467-019-12166-w. PubMed
Functional Enhancers Shape Extrachromosomal Oncogene Amplifications. Morton AR, Dogan-Artun N, Faber ZJ, MacLeod G, Bartels CF, Piazza MS, Allan KC, Mack SC, Wang X, Gimple RC, Wu Q, Rubin BP, Shetty S, Angers S, Dirks PB, Sallari RC, Lupien M, Rich JN, Scacheri PC. Cell. 2019 Nov 27;179(6):1330-1341.e13. doi: 10.1016/j.cell.2019.10.039. Epub 2019 Nov 21. PubMed
ASCL1 is a MYCN- and LMO1-dependent member of the adrenergic neuroblastoma core regulatory circuitry. Wang L, Tan TK, Durbin AD, Zimmerman MW, Abraham BJ, Tan SH, Ngoc PCT, Weichert-Leahey N, Akahane K, Lawton LN, Rokita JL, Maris JM, Young RA, Look AT, Sanda T. Nat Commun. 2019 Dec 9;10(1):5622. doi: 10.1038/s41467-019-13515-5. PubMed
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J. Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5. PubMed

Associated Plasmids

Targeting Oncogenic Super Enhancers in MYC-Dependent AML Using a Small Molecule Activator of NR4A Nuclear Receptors. Call SG, Duren RP, Panigrahi AK, Nguyen L, Freire PR, Grimm SL, Coarfa C, Conneely OM. Sci Rep. 2020 Feb 18;10(1):2851. doi: 10.1038/s41598-020-59469-3. PubMed
Noncoding Variants Connect Enhancer Dysregulation with Nuclear Receptor Signaling in Hematopoietic Malignancies. Li K, Zhang Y, Liu X, Liu Y, Gu Z, Cao H, Dickerson KE, Chen M, Chen W, Shao Z, Ni M, Xu J. Cancer Discov. 2020 May;10(5):724-745. doi: 10.1158/2159-8290.CD-19-1128. Epub 2020 Mar 18. PubMed
CRISPRi-mediated functional analysis of lung disease-associated loci at non-coding regions. Stuart WD, Guo M, Fink-Baldauf IM, Coleman AM, Clancy JP, Mall MA, Lim FY, Brewington JJ, Maeda Y. NAR Genom Bioinform. 2020 Jun;2(2):lqaa036. doi: 10.1093/nargab/lqaa036. Epub 2020 May 25. PubMed
Transcriptional repression of PTEN in neural cells using CRISPR/dCas9 epigenetic editing. Moses C, Hodgetts SI, Nugent F, Ben-Ary G, Park KK, Blancafort P, Harvey AR. Sci Rep. 2020 Jul 9;10(1):11393. doi: 10.1038/s41598-020-68257-y. PubMed
Silencing integrated SIV proviral DNA with TAR-specific CRISPR tools. Smith LM, Hodara VL, Parodi LM, Callery JE, Giavedoni LD. J Med Primatol. 2020 Oct;49(5):269-279. doi: 10.1111/jmp.12494. Epub 2020 Sep 9. PubMed
NFIA differentially controls adipogenic and myogenic gene program through distinct pathways to ensure brown and beige adipocyte differentiation. Hiraike Y, Waki H, Miyake K, Wada T, Oguchi M, Saito K, Tsutsumi S, Aburatani H, Yamauchi T, Kadowaki T. PLoS Genet. 2020 Sep 29;16(9):e1009044. doi: 10.1371/journal.pgen.1009044. eCollection 2020 Sep. PubMed
EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma. Shi X, Zheng Y, Jiang L, Zhou B, Yang W, Li L, Ding L, Huang M, Gery S, Lin DC, Koeffler HP. Nucleic Acids Res. 2020 Nov 18;48(20):11434-11451. doi: 10.1093/nar/gkaa901. PubMed
Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity. Sheppard HE, Dall'Agnese A, Park WD, Shamim MH, Dubrulle J, Johnson HL, Stossi F, Cogswell P, Sommer J, Levy J, Sharifnia T, Wawer MJ, Nabet B, Gray NS, Clemons PA, Schreiber SL, Workman P, Young RA, Lin CY. Cell Rep Med. 2021 Jan 19;2(1):100188. doi: 10.1016/j.xcrm.2020.100188. eCollection 2021 Jan 19. PubMed

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