Ophir Shalem Lab:
Shalem Lab plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214917 | TRE-mClover3-TDP-43ΔNLS | inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta |
| 214918 | EF1a-SRRD-HA (in pLEX_307) | constitutive expression of SRRD tagged with HA |
| 214919 | EF1a-SRRD-noTag (in pLEX_307) | constitutive expression of SRRD with no tag - used as control for SRRD-HA expression |
| 214920 | TRE-SRRD-mRuby3 | inducible expression of SRRD tagged on C-terminus with mRuby3 |
| 214921 | EF1a-SRRD-V5-APEX2 (in pLEX_307) | constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation |
| 214922 | TRE-mClover3 | expression vector control - contitutive expression of mClover3 alone |
| 214923 | TRE-mRuby3 | expression vector control - contitutive expression of mRuby33 alone |