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pTargetF Citations (22)

Plasmid Article: Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
PubMed Journal

Articles Citing pTargetF

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces. Huang H, Zheng G, Jiang W, Hu H, Lu Y. Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3. PubMed

Associated Plasmids

Growth rate control of flagellar assembly in Escherichia coli strain RP437. Sim M, Koirala S, Picton D, Strahl H, Hoskisson PA, Rao CV, Gillespie CS, Aldridge PD. Sci Rep. 2017 Jan 24;7:41189. doi: 10.1038/srep41189. PubMed
Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM. Liu X, Tian J, Liu L, Zhu T, Yu X, Chu X, Yao B, Wu N, Fan Y. Sci Rep. 2017 Jul 28;7(1):6786. doi: 10.1038/s41598-017-06988-1. PubMed
Making point mutations in Escherichia coli BL21 genome using the CRISPR-Cas9 system. Wang X, He J, Le K. FEMS Microbiol Lett. 2018 Jul 1;365(14). pii: 4955551. doi: 10.1093/femsle/fny060. PubMed
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH. Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x. PubMed

Associated Plasmids

CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis. Wu Y, Chen T, Liu Y, Lv X, Li J, Du G, Ledesma-Amaro R, Liu L. Metab Eng. 2018 Sep;49:232-241. doi: 10.1016/j.ymben.2018.08.012. Epub 2018 Aug 31. PubMed
BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Gordeeva J, Morozova N, Sierro N, Isaev A, Sinkunas T, Tsvetkova K, Matlashov M, Truncaite L, Morgan RD, Ivanov NV, Siksnys V, Zeng L, Severinov K. Nucleic Acids Res. 2019 Jan 10;47(1):253-265. doi: 10.1093/nar/gky1125. PubMed
Two-step pathway for isoprenoid synthesis. Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G. Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 10.1073/pnas.1812935116. PubMed

Associated Plasmids

Improving Cadmium Resistance in Escherichia coli Through Continuous Genome Evolution. Qin W, Zhao J, Yu X, Liu X, Chu X, Tian J, Wu N. Front Microbiol. 2019 Feb 20;10:278. doi: 10.3389/fmicb.2019.00278. eCollection 2019. PubMed
AcrAB-TolC Inhibition by Peptide-Conjugated Phosphorodiamidate Morpholino Oligomers Restores Antibiotic Activity in Vitro and in Vivo. Sturge CR, Felder-Scott CF, Pifer R, Pybus C, Jain R, Geller BL, Greenberg DE. ACS Infect Dis. 2019 Aug 9;5(8):1446-1455. doi: 10.1021/acsinfecdis.9b00123. Epub 2019 Jun 11. PubMed
RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli. Lee J, Lee DH, Jeon CO, Lee K. J Microbiol. 2019 Oct;57(10):910-917. doi: 10.1007/s12275-019-9354-6. Epub 2019 Sep 30. PubMed
Hydrogen Sulfide From Cysteine Desulfurase, Not 3-Mercaptopyruvate Sulfurtransferase, Contributes to Sustaining Cell Growth and Bioenergetics in E. coli Under Anaerobic Conditions. Wang J, Guo X, Li H, Qi H, Qian J, Yan S, Shi J, Niu W. Front Microbiol. 2019 Oct 11;10:2357. doi: 10.3389/fmicb.2019.02357. eCollection 2019. PubMed
Overexpression of AmpC Promotes Bacteriophage Lysis of Ampicillin-Resistant Escherichia coli. Wang S, Yin B, Yu L, Dang M, Guo Z, Yan G, Hu D, Gu J, Du C, Feng X, Han W, Adam YY, Sun C, Bosse JT, Lei L. Front Microbiol. 2020 Jan 8;10:2973. doi: 10.3389/fmicb.2019.02973. eCollection 2019. PubMed
Regulation of Microbial Metabolic Rates Using CRISPR Interference With Expanded PAM Sequences. Kim B, Kim HJ, Lee SJ. Front Microbiol. 2020 Feb 28;11:282. doi: 10.3389/fmicb.2020.00282. eCollection 2020. PubMed
Genetic editing of the virulence gene of Escherichia coli using the CRISPR system. Hou M, Sun S, Feng Q, Dong X, Zhang P, Shi B, Liu J, Shi D. PeerJ. 2020 Apr 6;8:e8881. doi: 10.7717/peerj.8881. eCollection 2020. PubMed
CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs. Lee HJ, Kim HJ, Lee SJ. Genome Res. 2020 May;30(5):768-775. doi: 10.1101/gr.257493.119. Epub 2020 Apr 23. PubMed
Real-time observation of CRISPR spacer acquisition by Cas1-Cas2 integrase. Budhathoki JB, Xiao Y, Schuler G, Hu C, Cheng A, Ding F, Ke A. Nat Struct Mol Biol. 2020 May;27(5):489-499. doi: 10.1038/s41594-020-0415-7. Epub 2020 May 4. PubMed
CRISPR-based curing and analysis of metabolic burden of cryptic plasmids in Escherichia coli Nissle 1917. Zainuddin HS, Bai Y, Mansell TJ. Eng Life Sci. 2019 Jun 3;19(6):478-485. doi: 10.1002/elsc.201900003. eCollection 2019 Jun. PubMed
Gene Knockout of Beneficial Plant-associated Bacillus spp. Using the CRISPR-Cas9 Double Plasmid System. Figueredo EF, Quecine MC. Methods Mol Biol. 2021;2232:173-191. doi: 10.1007/978-1-0716-1040-4_15. PubMed
Multi-Path Optimization for Efficient Production of 2'-Fucosyllactose in an Engineered Escherichia coli C41 (DE3) Derivative. Ni Z, Li Z, Wu J, Ge Y, Liao Y, Yuan L, Chen X, Yao J. Front Bioeng Biotechnol. 2020 Dec 3;8:611900. doi: 10.3389/fbioe.2020.611900. eCollection 2020. PubMed
Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells. Feng H, Guo J, Wang T, Zhang C, Xing XH. Nucleic Acids Res. 2021 Feb 22;49(3):1263-1277. doi: 10.1093/nar/gkaa1295. PubMed
Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression. Lee M, Ryu M, Joo M, Seo YJ, Lee J, Kim HM, Shin E, Yeom JH, Kim YH, Bae J, Lee K. PLoS Pathog. 2021 Feb 1;17(2):e1009263. doi: 10.1371/journal.ppat.1009263. eCollection 2021 Feb. PubMed

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