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Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH
Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113134E.coli_GW-sgRNA_Lib1Genome-wide gRNA library for E. coli, sub1
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113135E.coli_GW-sgRNA_Lib2Genome-wide gRNA library for E. coli, sub2
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113136E.coli_GW-sgRNA_Lib3Genome-wide gRNA library for E. coli, sub3
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113137E.coli_GW-sgRNA_Lib4Genome-wide gRNA library for E. coli, sub4
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113146E.coli_NC_sgRNA_libGenome-wide gRNA library for E. coli, non-targeting control
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113149pdCas9-J23111Plasmid constitutively expressing dCas9 protein
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