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Two-step pathway for isoprenoid synthesis.
Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G
Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 10.1073/pnas.1812935116. PubMed Article

Plasmids from Article

ID Plasmid Purpose
122017p5T7-LYCipi-ggppsExpresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
122018pSEVA228-pro4IUPiExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
122019pTET-IUPiExpresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

Antibodies from Article