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(Plasmid #62552)


Item Catalog # Description Quantity Price (USD)
Plasmid 62552 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression, CRISPR ; Bacterial Genome Editing

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    artificially synthesized, codon optimized gene from Strep pyogenes
  • Insert Size (bp)
  • Promoter ptipA

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer M13pUC-Rev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sgRNA ()
  • Insert Size (bp)
  • Promoter j23119

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    act-orf4 homology arm
  • Insert Size (bp)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer M13pUC-Fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKCcas9dO was a gift from Weihong Jiang (Addgene plasmid # 62552 ; ; RRID:Addgene_62552)
  • For your References section:

    One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces. Huang H, Zheng G, Jiang W, Hu H, Lu Y. Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3. 10.1093/abbs/gmv007 PubMed 25739462