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(Plasmid #61736)


Item Catalog # Description Quantity Price (USD)
Plasmid 61736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 11474
  • Modifications to backbone
    mutation G9173C to remove BbsI site from pSG5 rep
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    Streptomyces codon-optimized Cas9
  • Species
  • Insert Size (bp)
  • Promoter rpsLp(XC)-BbsI

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
  • Promoter rpsLp(CF)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    crRNA cassette
  • Species
  • Insert Size (bp)
  • Mutation
    BbsI-flanked lacZ cassette inserted in place of spacer
  • Promoter gapdhp(EL)

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-1 was a gift from Huimin Zhao (Addgene plasmid # 61736 ; ; RRID:Addgene_61736)
  • For your References section:

    High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. Cobb RE, Wang Y, Zhao H. ACS Synth Biol. 2014 Dec 8. 10.1021/sb500351f PubMed 25458909