Skip to main content
Holiday Schedule: Addgene will be closed November 24th & 25th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

TetO-FUW-OSKM Citations (51)

Plasmid Article: Reprogramming of murine and human somatic cells using a single polycistronic vector.
Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
PubMed Journal

Articles Citing TetO-FUW-OSKM

Articles
Determination of protein interactome of transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors. Gao Z, Cox JL, Gilmore JM, Ormsbee BD, Mallanna SK, Washburn MP, Rizzino A. J Biol Chem. 2012 Mar 30;287(14):11384-97. doi: 10.1074/jbc.M111.320143. Epub 2012 Feb 9. PubMed
Sirtuin 1 facilitates generation of induced pluripotent stem cells from mouse embryonic fibroblasts through the miR-34a and p53 pathways. Lee YL, Peng Q, Fong SW, Chen AC, Lee KF, Ng EH, Nagy A, Yeung WS. PLoS One. 2012;7(9):e45633. doi: 10.1371/journal.pone.0045633. Epub 2012 Sep 21. PubMed
Predicting stem cell fate changes by differential cell cycle progression patterns. Roccio M, Schmitter D, Knobloch M, Okawa Y, Sage D, Lutolf MP. Development. 2013 Jan 15;140(2):459-70. doi: 10.1242/dev.086215. Epub 2012 Nov 28. PubMed
Barcoding cells using cell-surface programmable DNA-binding domains. Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM. Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407. PubMed

Associated Plasmids

Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming. Christoforou N, Chellappan M, Adler AF, Kirkton RD, Wu T, Addis RC, Bursac N, Leong KW. PLoS One. 2013 May 21;8(5):e63577. doi: 10.1371/journal.pone.0063577. Print 2013. PubMed
Induced pluripotent stem cell-derived cardiac progenitors differentiate to cardiomyocytes and form biosynthetic tissues. Christoforou N, Liau B, Chakraborty S, Chellapan M, Bursac N, Leong KW. PLoS One. 2013 Jun 13;8(6):e65963. doi: 10.1371/journal.pone.0065963. Print 2013. PubMed
Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Abad M, Mosteiro L, Pantoja C, Canamero M, Rayon T, Ors I, Grana O, Megias D, Dominguez O, Martinez D, Manzanares M, Ortega S, Serrano M. Nature. 2013 Oct 17;502(7471):340-5. doi: 10.1038/nature12586. Epub 2013 Sep 11. PubMed
Matrix identity and tractional forces influence indirect cardiac reprogramming. Kong YP, Carrion B, Singh RK, Putnam AJ. Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474. PubMed

Associated Plasmids

Nonstochastic reprogramming from a privileged somatic cell state. Guo S, Zi X, Schulz VP, Cheng J, Zhong M, Koochaki SH, Megyola CM, Pan X, Heydari K, Weissman SM, Gallagher PG, Krause DS, Fan R, Lu J. Cell. 2014 Feb 13;156(4):649-62. doi: 10.1016/j.cell.2014.01.020. Epub 2014 Jan 30. PubMed
KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, Yamamoto T, Yamanaka S, Woltjen K. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12. PubMed

Associated Plasmids

Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis. Jiang Y, Du M, Wu M, Zhu Y, Zhao X, Cao X, Li X, Long P, Li W, Hu B. Stem Cells Dev. 2016 Jan 1;25(1):43-54. doi: 10.1089/scd.2015.0159. Epub 2015 Nov 10. PubMed
Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines. Chang EA, Tomov ML, Suhr ST, Luo J, Olmsted ZT, Paluh JL, Cibelli J. Sci Rep. 2015 Oct 20;5:15234. doi: 10.1038/srep15234. PubMed
Transcription factor-mediated reprograming of fibroblasts to hepatocyte-like cells. Pournasr B, Asghari-Vostikolaee MH, Baharvand H. Eur J Cell Biol. 2015 Dec;94(12):603-10. doi: 10.1016/j.ejcb.2015.10.003. Epub 2015 Oct 24. PubMed
Physical Interactions and Functional Coordination between the Core Subunits of Set1/Mll Complexes and the Reprogramming Factors. Yang Z, Augustin J, Hu J, Jiang H. PLoS One. 2015 Dec 21;10(12):e0145336. doi: 10.1371/journal.pone.0145336. eCollection 2015. PubMed
Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal. Grigor'eva EV, Shevchenko AI, Medvedev SP, Mazurok NA, Zhelezova AI, Zakian SM. Acta Naturae. 2015 Oct-Dec;7(4):56-69. PubMed
Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA. Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067. PubMed

Associated Plasmids

Pancreatic Endoderm-Derived from Diabetic Patient-Specific Induced Pluripotent Stem Cell Generates Glucose-Responsive Insulin-Secreting Cells. Rajaei B, Shamsara M, Fakhr Taha M, Massumi M, Sanati MH. J Cell Physiol. 2016 Jun 16. doi: 10.1002/jcp.25459. PubMed
TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming. Miles DC, de Vries NA, Gisler S, Lieftink C, Akhtar W, Gogola E, Pawlitzky I, Hulsman D, Tanger E, Koppens M, Beijersbergen RL, van Lohuizen M. Stem Cells. 2017 Jan;35(1):147-157. doi: 10.1002/stem.2453. Epub 2016 Jul 6. PubMed
A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos. Wang J, Li X, Wang L, Li J, Zhao Y, Bou G, Li Y, Jiao G, Shen X, Wei R, Liu S, Xie B, Lei L, Li W, Zhou Q, Liu Z. EMBO Rep. 2016 Oct;17(10):1452-1470. Epub 2016 Aug 5. PubMed
Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts. Syed KM, Joseph S, Mukherjee A, Majumder A, Teixeira JM, Dutta D, Pillai MR. J Cell Sci. 2016 Dec 15;129(24):4576-4591. doi: 10.1242/jcs.194035. Epub 2016 Nov 14. PubMed
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program. Daniel MG, Pereira CF, Bernitz JM, Lemischka IR, Moore K. J Vis Exp. 2016 Dec 16;(118). doi: 10.3791/54372. PubMed
Differential gene expression profiles in neurons generated from lymphoblastoid B-cell line-derived iPS cells from monozygotic twin cases with treatment-resistant schizophrenia and discordant responses to clozapine. Nakazawa T, Kikuchi M, Ishikawa M, Yamamori H, Nagayasu K, Matsumoto T, Fujimoto M, Yasuda Y, Fujiwara M, Okada S, Matsumura K, Kasai A, Hayata-Takano A, Shintani N, Numata S, Takuma K, Akamatsu W, Okano H, Nakaya A, Hashimoto H, Hashimoto R. Schizophr Res. 2017 Mar;181:75-82. doi: 10.1016/j.schres.2016.10.012. Epub 2016 Oct 27. PubMed
Using DNA Methylation Profiling to Evaluate Biological Age and Longevity Interventions. Petkovich DA, Podolskiy DI, Lobanov AV, Lee SG, Miller RA, Gladyshev VN. Cell Metab. 2017 Apr 4;25(4):954-960.e6. doi: 10.1016/j.cmet.2017.03.016. PubMed
Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique. Yunusova AM, Fishman VS, Vasiliev GV, Battulin NR. Open Biol. 2017 Apr;7(4). pii: 160311. doi: 10.1098/rsob.160311. PubMed
Electrophysiological Properties and Viability of Neonatal Rat Ventricular Myocyte Cultures with Inducible ChR2 Expression. Li Q, Ni RR, Hong H, Goh KY, Rossi M, Fast VG, Zhou L. Sci Rep. 2017 May 8;7(1):1531. doi: 10.1038/s41598-017-01723-2. PubMed
Glucose Can Epigenetically Alter the Gene Expression of Neurotrophic Factors in the Murine Brain Cells. Hossain MS, Oomura Y, Katafuchi T. Mol Neurobiol. 2017 May 13. doi: 10.1007/s12035-017-0578-3. PubMed
Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional Hydrogels. Caiazzo M, Tabata Y, Lutolf M. Methods Mol Biol. 2017;1612:65-78. doi: 10.1007/978-1-4939-7021-6_5. PubMed
Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. Hong X, Margariti A, Le Bras A, Jacquet L, Kong W, Hu Y, Xu Q. Sci Rep. 2017 Jul 17;7(1):5590. doi: 10.1038/s41598-017-05665-7. PubMed
Reprogramming Factors Remodel Melanoma Cell Phenotype by Changing Stat3 Expression. Wang Y, Mou Y, Zhang H, Wang X, Li R, Cheng Z, Liu X. Int J Med Sci. 2017 Nov 2;14(13):1402-1409. doi: 10.7150/ijms.21952. eCollection 2017. PubMed
Microenvironment dependent gene expression signatures in reprogrammed human colon normal and cancer cell lines. Strainiene E, Binkis M, Urnikyte S, Stankevicius V, Sasnauskiene A, Kundrotas G, Kazlauskas A, Suziedelis K. BMC Cancer. 2018 Feb 27;18(1):222. doi: 10.1186/s12885-018-4145-8. PubMed
The Histone Variant MacroH2A Blocks Cellular Reprogramming by Inhibiting Mesenchymal-to-Epithelial Transition. Pliatska M, Kapasa M, Kokkalis A, Polyzos A, Thanos D. Mol Cell Biol. 2018 Apr 30;38(10). pii: MCB.00669-17. doi: 10.1128/MCB.00669-17. Print 2018 May 15. PubMed
A systems mechanobiology model to predict cardiac reprogramming outcomes on different biomaterials. Kong YP, Rioja AY, Xue X, Sun Y, Fu J, Putnam AJ. Biomaterials. 2018 Oct;181:280-292. doi: 10.1016/j.biomaterials.2018.07.036. Epub 2018 Jul 28. PubMed
SIRT2 is required for efficient reprogramming of mouse embryonic fibroblasts toward pluripotency. Kim AY, Lee EM, Lee EJ, Kim JH, Suk K, Lee E, Hur K, Hong YJ, Do JT, Park S, Jeong KS. Cell Death Dis. 2018 Aug 30;9(9):893. doi: 10.1038/s41419-018-0920-3. PubMed
Glucose-Responsiveness of Pancreatic beta-Like (GRP beta-L) Cells Generated from Human Pluripotent Stem Cells. Rajaei B, Massumi M, Wheeler M. Curr Protoc Hum Genet. 2019 Jan;100(1):e71. doi: 10.1002/cphg.71. Epub 2018 Oct 18. PubMed
Establishment of stably expandable induced myogenic stem cells by four transcription factors. Lee EJ, Kim M, Kim YD, Chung MJ, Elfadl A, Ulah HMA, Park D, Lee S, Park HS, Kim TH, Hwang D, Jeong KS. Cell Death Dis. 2018 Oct 25;9(11):1092. doi: 10.1038/s41419-018-1114-8. PubMed
Immortalized murine fibroblast cell lines are refractory to reprogramming to pluripotent state. Skvortsova EV, Sinenko SA, Tomilin AN. Oncotarget. 2018 Oct 16;9(81):35241-35250. doi: 10.18632/oncotarget.26235. eCollection 2018 Oct 16. PubMed
Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Wang Y, Sun HJ, Li RG, Wang XM, Cheng ZQ, Lou N. Mol Med Rep. 2019 Feb;19(2):967-973. doi: 10.3892/mmr.2018.9753. Epub 2018 Dec 12. PubMed
Wild-Type p53 Promotes Cancer Metabolic Switch by Inducing PUMA-Dependent Suppression of Oxidative Phosphorylation. Kim J, Yu L, Chen W, Xu Y, Wu M, Todorova D, Tang Q, Feng B, Jiang L, He J, Chen G, Fu X, Xu Y. Cancer Cell. 2019 Feb 11;35(2):191-203.e8. doi: 10.1016/j.ccell.2018.12.012. Epub 2019 Jan 31. PubMed
Chemical screen for epigenetic barriers to single allele activation of Oct4. Headley KM, Kedziora KM, Alejo A, Lai EZ, Purvis JE, Hathaway NA. Stem Cell Res. 2019 Jul;38:101470. doi: 10.1016/j.scr.2019.101470. Epub 2019 May 24. PubMed
Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Khoshchehreh R, Totonchi M, Carlos Ramirez J, Torres R, Baharvand H, Aicher A, Ebrahimi M, Heeschen C. Oncogene. 2019 Aug;38(34):6226-6239. doi: 10.1038/s41388-019-0871-x. Epub 2019 Jul 15. PubMed
A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State. Sherstyuk VV, Davletshina GI, Vyatkin YV, Shtokalo DN, Vlasov VV, Zakian SM. Acta Naturae. 2019 Apr-Jun;11(2):92-97. doi: 10.32607/20758251-2019-11-2-92-97. PubMed
Glutamine independence is a selectable feature of pluripotent stem cells. Vardhana SA, Arnold PK, Rosen BP, Chen Y, Carey BW, Huangfu D, Carmona Fontaine C, Thompson CB, Finley LWS. Nat Metab. 2019 Jul;1(7):676-687. doi: 10.1038/s42255-019-0082-3. Epub 2019 Jul 8. PubMed
Sox2 and Klf4 as the Functional Core in Pluripotency Induction without Exogenous Oct4. An Z, Liu P, Zheng J, Si C, Li T, Chen Y, Ma T, Zhang MQ, Zhou Q, Ding S. Cell Rep. 2019 Nov 12;29(7):1986-2000.e8. doi: 10.1016/j.celrep.2019.10.026. PubMed
Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Ruiz G, Valencia-Gonzalez HA, Perez-Montiel D, Munoz F, Ocadiz-Delgado R, Fernandez-Retana J, Perez-Plasencia C, Resendis-Antonio O, Gariglio P, Garcia-Carranca A. Stem Cells Int. 2019 Dec 1;2019:7683817. doi: 10.1155/2019/7683817. eCollection 2019. PubMed
CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. Xu S, Kim J, Tang Q, Chen Q, Liu J, Xu Y, Fu X. Protein Cell. 2020 May;11(5):352-365. doi: 10.1007/s13238-020-00699-6. Epub 2020 Mar 13. PubMed
The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion. Nagai Y, Takayama K, Nishitani N, Andoh C, Koda M, Shirakawa H, Nakagawa T, Nagayasu K, Yamanaka A, Kaneko S. Int J Mol Sci. 2020 Mar 21;21(6). pii: ijms21062160. doi: 10.3390/ijms21062160. PubMed
Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury. Wang S, Tian X, Li Y, Xue R, Guan H, Lu M, Xu H, Ye Z, Chen S, Xiang M. Oxid Med Cell Longev. 2020 Mar 26;2020:1609638. doi: 10.1155/2020/1609638. eCollection 2020. PubMed
Sirt1 is regulated by miR-135a and involved in DNA damage repair during mouse cellular reprogramming. Chen ACH, Peng Q, Fong SW, Yeung WSB, Lee YL. Aging (Albany NY). 2020 Apr 26;12(8):7431-7447. doi: 10.18632/aging.103090. Epub 2020 Apr 26. PubMed
MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation. Batlle C, Calvo I, Iglesias V, J Lynch C, Gil-Garcia M, Serrano M, Ventura S. Commun Biol. 2021 Mar 26;4(1):414. doi: 10.1038/s42003-021-01930-8. PubMed
Enhanced chromatin accessibility contributes to X chromosome dosage compensation in mammals. Talon I, Janiszewski A, Theeuwes B, Lefevre T, Song J, Bervoets G, Vanheer L, De Geest N, Poovathingal S, Allsop R, Marine JC, Rambow F, Voet T, Pasque V. Genome Biol. 2021 Nov 1;22(1):302. doi: 10.1186/s13059-021-02518-5. PubMed
MYCL-mediated reprogramming expands pancreatic insulin-producing cells. Hirano M, So Y, Tsunekawa S, Kabata M, Ohta S, Sagara H, Sankoda N, Taguchi J, Yamada Y, Ukai T, Kato M, Nakamura J, Ozawa M, Yamamoto T, Yamada Y. Nat Metab. 2022 Feb;4(2):254-268. doi: 10.1038/s42255-022-00530-y. Epub 2022 Feb 10. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.