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Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation.
McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA
Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067. PubMed Article

Plasmids from Article

ID Plasmid Purpose
78254TetO-dCas9-D3AExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
78255TetO-dCas9-mD3AExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
78256pCMV-dCas9-D3AExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter
78257pCMV-dCas9-mD3AExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

Antibodies from Article