We narrowed to 18,413 results for: multi

1. pSLiP-G1G2

   Plasmid

   #188965

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PrhaBAD

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

   Plasmid

   #186413

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli)
   

   Use

   Gateway destination vector

   Tags

   Nuclear localization signal

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

   Plasmid

   #127776

   Purpose

   Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   tfap2b (TFAP2B Chicken)
   

   Use

   Unspecified

   Tags

   Avi

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB1C3_mTq-Tat (c-plasmid)

   Plasmid

   #127635

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   mTurquoise2
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PCP and RNA binding peptide:…

   Expression

   Bacterial

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111922

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EcNGRfC

   Bacterial Strain

   #48936

   Purpose

   Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)

   Depositor

   George Church

   Bacterial Resistance

   Spectinomycin

   Available Since

   Dec. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. EcNGRfEfC

   Bacterial Strain

   #48937

   Purpose

   Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)

   Depositor

   George Church

   Bacterial Resistance

   Spectinomycin

   Available Since

   Dec. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Expression

   Bacterial

   Promoter

   PosmY, PsulA, and grpE

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Promoter

    EF1a

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY108 (lenti-AsCpf1)

    Plasmid

    #84739

    Purpose

    Lenti virus delivery of AsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Promoter

    EFS

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2R3a-TagRFP-OcsT

    Plasmid

    #71270

    Purpose

    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Promoter

    EFS

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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