We narrowed to 8,256 results for: pcDNA3.1+

1. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

   Plasmid

   #69906

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-KozATG-dL5-2XG4S-actin

   Plasmid

   #73262

   Purpose

   Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

   Insert

   KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
   

   Use

   Tags

   The FAP and actin are fused with 2 copies of a G4…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

   Plasmid

   #86885

   Purpose

   C-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   BirA* (AA141-321)- HA- NIPP1]
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 141-321

   Promoter

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 CMV mCherry-eGFP BGH

   Plasmid

   #92280

   Purpose

   Control FRET construct; tandem mCherry-eGFP fusion

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   mCherry-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

   Plasmid

   #114111

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

   Insert

   Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/nV5-DEST-beta catenin

   Plasmid

   #20140

   Depositor

   Valeri Vasioukhin

   Article

   Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

   Insert

   pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   T3I

   Promoter

   CMV

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/FGFR2-S252W(Apert) myc

   Plasmid

   #33249

   Depositor

   Claudio Basilico, Alka Mansukhani

   Article

   Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

   Insert

   FGFR2 (Fgfr2 Mouse)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Mutation S252W in FGFR2 found in Apert syndrome. …

   Promoter

   CMV

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/CIP2A(1-905) L533E V5 His

    Plasmid

    #119288

    Purpose

    CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    L533E

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1 Flag-HIF1B [F446L] (#931)

    Plasmid

    #99917

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F446L

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NC3 pCDNA3.1 FLAG WTX (1-804)

    Plasmid

    #36958

    Depositor

    Randall Moon

    Article

    Major et al Science. 2007 May 18;316(5827):1043-6.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

    Plasmid

    #127312

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

    Plasmid

    #69900

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (stw, CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge