We narrowed to 22,515 results for: tes

1. LoxP3-Mb2-HA-mTFP1-2A (SO157)

   Plasmid

   #99615

   Purpose

   To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2-HA-mTfp1
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBP2-like-bio

   Plasmid

   #68536

   Purpose

   Expresses full-length P. vivax RBP2-like (PVX_101590) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   RBP2-like
   

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-yHS1-M7A,H102A

   Plasmid

   #159165

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A,H102A
   

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pT2KXIG (Tol2)-tp63:ikk1C179A:GFP

   Plasmid

   #105647

   Purpose

   Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   tp63:ikk1C179A:GFP (chuk Zebrafish)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p63 promoter + ikk1C179A + GFP

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rat SERCA2b (pMT2)

   Plasmid

   #75184

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   Jonathan Lytton, Gary Shull

   Article

   Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

   Insert

   ATP2A2
   

   Expression

   Mammalian

   Promoter

   adenovirus major late promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRT2-H2B-EGFP-V5-2A (IG157)

   Plasmid

   #99623

   Purpose

   To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   H2B-EGFP-V5
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRT3-Mb2-HA-mTfp1-2A (IG155)

   Plasmid

   #99621

   Purpose

   To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2-HA-mTfp1
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRT1-Mb2YFP-2A (IG153)

   Plasmid

   #99619

   Purpose

   To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2EYFP
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRT2-Mb2Tomato-2A (IG154)

   Plasmid

   #99620

   Purpose

   To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2Tomato
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRT3-HA-H2B-Cerulean-2A (IG158))

    Plasmid

    #99624

    Purpose

    To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-RUNX1-4A

    Plasmid

    #238534

    Purpose

    Used for overexpression of human RUNX1 in mammalian cells. Contains mutations that prevent phosphorylation at some sites of the translated protein.

    Depositor

    Diane Krause

    Article

    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

    Inserts

    RUNX1 (RUNX1 Human)
    Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S249A, S266A, T273A, S276A

    Promoter

    MSCV and PGK

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC32B-AtmiR173aTS-B/c

    Plasmid

    #227965

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Expression

    Plant

    Promoter

    35S and None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-NbmiR482aTS-B/c

    Plasmid

    #227966

    Purpose

    Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

    Plasmid

    #206178

    Purpose

    Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Cre
    

    Use

    AAV

    Promoter

    cTnT

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-2T-TRR-C421-G2304D

    Plasmid

    #182845

    Purpose

    mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    mutation G2304D (GGC/GAC)

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-2T-TRX-C751-E3616S

    Plasmid

    #182844

    Purpose

    mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRX (w Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgFFL_7x_Bulge

    Plasmid

    #59894

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has seven bulge targets (fully comple…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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