... CRISPR Parasites CRISPR Plasmids: Parasites Browse CRISPR Plasmids ...Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such as Plasmodium...been designed for use in parasites. CRISPR...propelled both reverse and forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also...also been established for some of these parasites. Together, these tools have enabled precise genetic ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

...for serotype testing: AAV1, AAV2, AAV5, AAV8 and AAV9... Viral Service AAV Serotype Testing AAV Viral Service...Service: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism...Inventory . The catalog ID for the 20 µL sized testing sample is denoted with a .T suffix (for example... (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use...

...expressing secreted recombinant proteins from human erythrocytes that was created for systematic screening of...

...serotype testing panel for the eye and brain.... Viral Service AAV University of Florida Serotype Testing Panel... Viral Service: University of Florida Serotype Testing Panel for the Eye and Brain You may also like: ...by ddPCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...injection in mice and macaque. This serotype also demonstrates enhanced transduction of cells in culture for... with improved mGluR6 promoters in rodents and primates. Mol Ther . 2016 Aug;23(8-9):680-9. PMID: 27115727...

...Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat. Methods . 2013 July. PubMed PMID...trans from pSPgRNA, the dCas9-VP64 fusion protein activates the expression of downstream genes. Multiple sgRNAs...

...TAAAGACTCCTCCACCAGccaaacctgcggccccTGCAGTGTCCAGTAGTGA tes TAL3190 & TAL3191 TTGAGGACCATGACGTGCagctggagactgaagaGAGCAAGAAAGTTGGGAA... Joung, Peterson, and Yeh labs at MGH to target sites within various endogenous zebrafish genes; many ...Gene Name TALENs Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209...

...GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016...recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition..., 2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic...portion of the ORF that is sandwiched between the sites; the starting direction of these ORF fragments (...design. How-to guide for the molecular design and testing of novel INTSRECT plasmids for groups interested...targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre... recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO EYFP Flp 55640...

... μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100%...100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized...the column using water or a neutral buffer such as TE. You will now have plasmid DNA that has been purified... Water-saturated phenol-chloroform can be used if TE-saturated is not available. Vortex microfuge tube... paper towel for 5-20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). *Pro-Tip* DNA... and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration of clean plasmid...

...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. *Pro-Tip* Be sure to swirl your plates after pouring...

...Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes,...residual ethanol wash and add RNase-free water or TE as soon as the entire tube is dried but while the...visible. Resuspend RNA pellet in RNase-free water or TE. Quantify and assess the quality of your RNA sample...Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000 × g at 4°C ...Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000 × g at 4°C to separate...

...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...

...new target sites. Created in BioRender.com.   Class II TEs: DNA transposons Class II TEs are also ...of transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons.... Class I Transposable elements (TEs): Retrotransposons  Class I TEs are also known as retrotransposons...the TE while also increasing the size of their host’s genome.   There are two types of Class 1 TEs: those...DNA sequences or target sites. Integration of most transposable elements (TEs) results in the duplication...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...encode a transposase enzyme.  Class I TEs are considered replicative since every time they jump they make...

.... We asked all of the Addgenies to pick their favorites out of the bunch and here’s what ended up on top...stopped working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff... of our entrants had fun decorating with their labmates! ...

...everyone who participated in our annual #Deckthelab contest. The bar was set very high from last year’s impressive...chocolate for the lab. Here are some of our 2017 contest winners:   Chicagoland's Community Biology ...Stanford University was so moved by our deck the lab contest that she spent a year hoarding used tip boxes for...lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament ...blast decorating and creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners...winners? Be sure to enter our 2018 #Deckthelab contest when the next holiday season rolls around! ...

...annual Deck The Lab holiday decorating contest! Our #DeckTheLab contest is a time for scientists worldwide...the details:   Contest 1: Deck the Lab - Classic Edition This edition of the contest is our traditional...December 23, 2020 Contest 2: Deck the Lab - Home Edition This edition of the contest should showcase a...for either contest on Twitter or Instagram by December 31, 2021. Let us know which contest you are entering...celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter and Instagram as... show off their decorating skills. To join the contest, simply embellish and adorn your lab space or your.... Have fun! Here’s an example from last year’s contest:   🎶Walking in a Winter Wonderlab🎶 We decided...

...non-parametric test may be the way to go, which we will discuss more in another blog. T-tests T-tests compare...classic parametric tests and one of the simplest statistical tests available. Tailed t-tests If you are solely...ANOVA tests are essentially t-tests with room for more variables and groups. ANOVAs can test three or more...within a test, depends on the input data ‘following the rules’ of that test. All statistical tests have assumptions...distribution profiles.   Parametric tests  Statistical tests fall into two general categories: parametric... blog we will be discussing parametric tests. Parametric tests are suitable for data sets that “play by...need to be met for a parametric test are as follows:   Parametric test assumptions: Distribution – Normal...

...considerations, and give just the lightest of nods towards conjugates.  Storage   Once you get your exciting...antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the conjugates, or signaling...questions is “yes,” then I would suggest either testing the antibody efficacy through a standard curve,...buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many commercial...much every other assay you can do. Most non-HRP conjugates are fluorophores, which work not by reacting ...

...of the Federal Labor Standards Act (FLSA) which mandates that all workers across the country either be ...specifically chosen to better define productive routes for advocacy efforts and help participants develop...Fisher Scientific Developing effective science advocates is an important way to push the balance from challenge... advocates...

...learn more! Background: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in... RNA. Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...structure can affect editing efficiency. Gao et al. tested if NgAgo preferred certain guide sequences - using...