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pCK353
Plasmid#129697PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120015170
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK355
Plasmid#129699PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminatorDepositorInsertsPrhaBAD
yfp
rhaS
ilvBN terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
FB027
Plasmid#119712PurposeMultipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMTDepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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FB039
Plasmid#119715PurposeMultipartite assembly obtained by combining FBparts FB026+FB009 into pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance + YFP), according to FungalBraid modular DNA assembly for ATMT.DepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH003
Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3C
Plasmid#89292PurposeFor expression of the mammalian Atg8 protein LC3C without a tagDepositorInsertLC3C (MAP1LC3C Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC32 [TU#839]
Plasmid#16079DepositorInsertmec-2 intron 9
UseTagsYFPExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
C11m
Plasmid#120959PurposeMoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCitrine
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIMY
Plasmid#191954PurposeA pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFPDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MEIS1A-MIY
Plasmid#21013DepositorInsertMEIS1A (Meis1 Mouse)
UseRetroviralTagsIRES and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL1
Plasmid#89294PurposeFor expression of the mammalian Atg8 protein GABARAPL1 without a tagDepositorInsertGABARAPL1 (GABARAPL1 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL2
Plasmid#89298PurposeFor expression of the mammalian Atg8 protein GABARAPL2 tagged with HADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsHA and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL1
Plasmid#89297PurposeFor expression of the mammalian Atg8 protein GABARAPL1 tagged with HADepositorInsertGABARAPL1 (GABARAPL1 Human)
UseRetroviralTagsHA and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3A
Plasmid#89290PurposeFor expression of the mammalian Atg8 protein LC3A without a tagDepositorInsertLC3A (MAP1LC3A Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3B
Plasmid#89291PurposeFor expression of the mammalian Atg8 protein LC3B without a tagDepositorInsertLC3B (MAP1LC3B Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only
