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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,023 results for: golden gate

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Showing: 3061 - 3080 of 5023 results

  1. pNM3746

    Plasmid
    #154109
    Purpose
    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    Use
    Cre/Lox; Rmce
    Expression
    Worm
    Promoter
    not applicable
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154109-plasmid-map-sequence-id-306068

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  3. pEPOR1CB0113

    Plasmid
    #117552
    Purpose
    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117552-plasmid-map-sequence-id-228090

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  6. pTU2_2a

    Plasmid
    #73010
    Purpose
    Secondary module (BpiI) site in pTU2a backbone
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Secondary module + Golden Gate pTU2a BsmBI destination site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    73010-plasmid-map-sequence-id-133861

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  14. M_3HN5HN

    Plasmid
    #249780
    Purpose
    Minimal synthetic construct for assessing speckle localization containing a 3' splice site, HNRNP motif-rich region, 5' splice site, and a second HNRNP motif-rich region
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_3HN5HN
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249780-plasmid-map-sequence-id-508117

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  15. M_3SR5SR

    Plasmid
    #249779
    Purpose
    Minimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, 5' splice site, and a second SR motif-rich region
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_3SR5SR
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249779-plasmid-map-sequence-id-508110

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  16. pSL187

    Plasmid
    #252926
    Purpose
    Anhydrotetracycline (aTc) sensing in the sender Lactococcus lactis with RBSLl1 for use in e-COSENS
    Depositor
    Caroline Ajo-Franklin
    Article
    Li et al Nat Biotechnol (2026).
    Insert
    menD
    Expression
    Bacterial
    Promoter
    PtetR-D7_Pxyl/2xtetO
    Available Since
    April 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    252926-plasmid-map-sequence-id-503434

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  17. pSL186

    Plasmid
    #252927
    Purpose
    Anhydrotetracycline (aTc) sensing in the sender Lactococcus lactis with RBSLl2 for use in e-COSENS
    Depositor
    Caroline Ajo-Franklin
    Article
    Li et al Nat Biotechnol (2026).
    Insert
    menD
    Expression
    Bacterial
    Promoter
    PtetR-D7_Pxyl/2xtetO
    Available Since
    April 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    252927-plasmid-map-sequence-id-503638

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Showing: 3061 - 3080 of 5023 results