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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,805 results for: pcr

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Showing: 3061 - 3080 of 7805 results

  4. pACT5FVMF(f)

    Plasmid
    #110549
    Purpose
    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
    Depositor
    David Russell
    Article
    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10
    Insert
    EGFP, FV LTRs
    Use
    Qpcr control for titering foamy virus
    Promoter
    promoter: MLV LTR
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110549-plasmid-map-sequence-id-212295

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  17. MRGPRX4-Tango

    Plasmid
    #66442
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX4 (MRGPRX4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. MRGPRX1-Tango

    Plasmid
    #66439
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. SSTR2-DuET

    Plasmid
    #213377
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR2 (SSTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213377-plasmid-map-sequence-id-430733

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Showing: 3061 - 3080 of 7805 results