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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-TMEM9-EGFP

Plasmid

#225515

Purpose

Lentiviral TMEM9-EGFP expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM9 (TMEM9 Human)

Use

Lentiviral

Tags

EGFP

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOT1T2

Plasmid

#225055

Purpose

Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Mutation

WT

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.6t

Plasmid

#210258

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mScarlet
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR

Plasmid

#193811

Purpose

CMV overexpression of ChemoR in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR

Tags

10xHis

Expression

Mammalian

Mutation

mScarlet: D201K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoR-CaM

Plasmid

#193815

Purpose

Protein production of ChemoR-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-CaM

Tags

10xHis

Expression

Bacterial

Mutation

mScarlet: D201K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-145

Plasmid

#185934

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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IBMc330

Plasmid

#161952

Purpose

Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA171-PSrpR-B30-mScarlet-I-Ter

Expression

Bacterial

Promoter

PSrpR

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Cerulean

Plasmid

#127771

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE2

Use

Enhancer reporter construct

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-mRFP

Plasmid

#117787

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETA-Prp28

Plasmid

#111285

Purpose

Prp28 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP28

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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ZBTB21_pCSdest

Plasmid

#53925

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ZBTB21

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
C21orf49_pCSdest

Plasmid

#53846

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

C21orf49

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGCS-3xFlag-ATP11B

Plasmid

#225511

Purpose

ATP11B expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

ATP11B (ATP11B Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-OXER1-mKate2

Plasmid

#226713

Purpose

overexpression red fluorescent OXER1 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

OXER1 (OXER1 Human)

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEG302-ELF7-ZF

Plasmid

#200920

Purpose

Express ELF7-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

ELF7 (ELF7 Mustard Weed)

Expression

Plant

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_RV-B14_VP1

Plasmid

#202074

Purpose

Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

RV-B14 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_RV-A89_VP1

Plasmid

#202073

Purpose

Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

RV-A89 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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