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We narrowed to 3,637 results for: golden gate

Showing: 3121 - 3140 of 3637 results

  1. TL1 alphaDdCBE 1-2 (right, DddA11-N)

    Plasmid
    #222000
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222000-plasmid-map-sequence-id-438640

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  2. TL1 alphaDdCBE 2 (left, DddA11-C)

    Plasmid
    #222001
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-2 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222001-plasmid-map-sequence-id-438748

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  3. TL1 alphaDdCBE 3_CN (left, DddA11-C)

    Plasmid
    #222002
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222002-plasmid-map-sequence-id-438715

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  4. TL1 alphaDdCBE 3_CN (right, DddA11-N)

    Plasmid
    #222003
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222003-plasmid-map-sequence-id-438752

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  5. TL1 alphaDdCBE 3_NC (left, DddA11-N)

    Plasmid
    #222004
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222004-plasmid-map-sequence-id-443120

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  6. pTE5423_p3WJdB-glcO60

    Plasmid
    #221194
    Purpose
    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO60-3WJdB
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter with downstream glcO60 operator
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221194-plasmid-map-sequence-id-441878

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  7. pro::EX1-2(intΔNACs&ΔMYBs):GFP

    Plasmid
    #218572
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218572-plasmid-map-sequence-id-437877

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  8. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Article
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Use
    Tags
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Promoter
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  18. pAL005

    Plasmid
    #233865
    Purpose
    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Depositor
    Jeff Hasty
    Article
    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
    Insert
    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    233865-plasmid-map-sequence-id-468718

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  19. pBWB514

    Plasmid
    #209326
    Purpose
    p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.
    Depositor
    Adam Arkin
    Article
    Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/
    Insert
    mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tet
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    209326-plasmid-map-sequence-id-414115

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Showing: 3121 - 3140 of 3637 results