We narrowed to 3,637 results for: golden gate

1. pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

   Plasmid

   #235327

   Purpose

   PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a (human EF1a)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235268

   Purpose

   Lentiviral expression of ComMAND base gene regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235317

   Purpose

   Lentiviral expression of mRuby2 with intronic microRNA only

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMDC32B-B/c

   Plasmid

   #227963

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHW1385

   Plasmid

   #229879

   Purpose

   Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
   

   Use

   Tags

   mScarlet-I-C1

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIE324-ZF6x12-C DsRed-Express2 in TUPV1

   Plasmid

   #201536

   Purpose

   DsRed-Express2 reporter regulated by ZF6 synTF promoter (12x compact binding sites) in TUPV1 for mMoClo golden gate-based assembly

   Depositor

   Joshua Leonard

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   DsRed-Express2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF6x12(C)_YB_TATA Minimal Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

   Plasmid

   #201537

   Purpose

   Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene

   Depositor

   Joshua Leonard

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   Promoterless PuroR-P2A-miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pR-mOrange2

   Plasmid

   #228608

   Purpose

   Mammalian expression of mOrange2, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pR-mBeRFP

   Plasmid

   #228601

   Purpose

   Mammalian expression of mBeRFP, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mBeRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR-mTagBFP2

    Plasmid

    #228596

    Purpose

    Mammalian expression of mTagBFP2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR-mT-Sapphire

    Plasmid

    #228597

    Purpose

    Mammalian expression of mT-Sapphire, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pR-LSSmOrange

    Plasmid

    #228600

    Purpose

    Mammalian expression of LSSmOrange, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    LSSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pR-mTFP1

    Plasmid

    #228602

    Purpose

    Mammalian expression of mTFP1, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pR-mRuby3

    Plasmid

    #228609

    Purpose

    Mammalian expression of mRuby3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pM-mT-Sapphire

    Plasmid

    #228616

    Purpose

    Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUS-miRFP670-TRF1

    Plasmid

    #227378

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric FUS IDR, WT (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUS-9YS-miRFP670-TRF1

    Plasmid

    #227381

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric FUS IDR, 9YS (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    9YS

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUS-27YS-miRFP670-TRF1

    Plasmid

    #227383

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric FUS IDR, 27YS (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    27YS

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. iLID-miRFP670-TRF1

    Plasmid

    #227385

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric iLID (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUS-15YS-miRFP670-TRF1

    Plasmid

    #227382

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric FUS IDR, 15YS (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    15YS

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge