Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,538 results for: golden gate

Showing: 3181 - 3200 of 5538 results

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35S-ZFN-pDGB3alpha1

Plasmid

#240729

Purpose

Encodes a ZFN that targets the broken GUS:NPTII reporter.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

ZFN

Expression

Plant

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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p2KanEG

Plasmid

#237279

Purpose

Empty vector for L2 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Plac

Plasmid

#243921

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Para

Plasmid

#243923

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, not including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Plac

Plasmid

#243910

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Para

Plasmid

#243912

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Ptet

Plasmid

#243914

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTampFRT

Plasmid

#243898

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTkanFRT

Plasmid

#243899

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTspcFRT

Plasmid

#243902

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_FRTcamFRT

Plasmid

#243836

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cat

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_FRTtetFRT

Plasmid

#243837

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_FRTspcFRT

Plasmid

#243838

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_FRTampFRT

Plasmid

#243839

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_FRTkanFRT

Plasmid

#243840

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_FRTcamFRT

Plasmid

#243841

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cat

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_FRTspcFRT

Plasmid

#243843

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_FRTampFRT

Plasmid

#243834

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAH120

Plasmid

#248733

Purpose

PBT1004 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT1004-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAH108

Plasmid

#248728

Purpose

PBT0463 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0463-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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