We narrowed to 3,637 results for: golden gate

1. FUS-5YS-miRFP670-TRF1

   Plasmid

   #227380

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric FUS IDR, 5YS (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   5YS

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FUS-3YS-miRFP670-TRF1

   Plasmid

   #227379

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric FUS IDR, 3YS (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   3YS

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJB50

   Plasmid

   #226311

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

   Plasmid

   #221994

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTE5418_pMBP-GlcR

   Plasmid

   #221192

   Purpose

   Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Insert

   glcR (pden4400 from Paracoccus denitrificans)
   

   Use

   Tags

   10x His tag with E. coli maltose binding protein …

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter and T7 promoter-lacO

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTE5419_p3WJdB-glcO36

   Plasmid

   #221193

   Purpose

   GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Insert

   T7 promoter-glcO36
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter with downstream glcO36 operator

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Gly-tRNA-gRNA scaffold for library 2

    Plasmid

    #192365

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Pro-tRNA-gRNA scaffold for library 2

    Plasmid

    #192366

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Gly-tRNA-gRNA scaffold for library 1

    Plasmid

    #192368

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Pro-tRNA-gRNA scaffold for library 1

    Plasmid

    #192369

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0KN0751

    Plasmid

    #185628

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mt-DdCBE G1397-C

    Plasmid

    #174563

    Purpose

    mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-C
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mt-DdCBE G1397-N

    Plasmid

    #174562

    Purpose

    mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-N
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cp-DdCBE G1397-C

    Plasmid

    #174559

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-C
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cp-DdCBE G1397-N

    Plasmid

    #174558

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-N
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EC71102

    Plasmid

    #154065

    Purpose

    Level 0 Golden Gate vector; Unmodifed CRE

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    CRE
    

    Use

    Synthetic Biology; Standard cloning vector used b…

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICSL11062

    Plasmid

    #68256

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_AtU6-26 + sgRNA_BolC.GA4a-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge