Modular gene tagging in C. elegans.
Hefel A, Kruse K, Wall K, Jorgensen SB, Ng KH, Stolley R, Rich MS, Jorgensen EM
bioRxiv [Preprint]. 2026 Jan 15:2026.01.15.699741. doi: 10.64898/2026.01.15.699741.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 259315 | pAJH116 | Germline FLP for injection |
| 259316 | pAJH120 | B3 destination vector for PhIT transgene insertions |
| 259318 | pAJH15 | Germline B3 for injection |
| 259319 | pAJH155 | PhIT tag B3RT2 n-term mKate2 |
| 259321 | pAJH160 | PhIT tag B3RT1 c-term GFP::stop |
| 259322 | pAJH238 | PhIT tag rox n-term 7xGFP11 |
| 259323 | pAJH256 | PhIT tag rox c-term 7xGFP11::stop |
| 259325 | pAJH27 | Germline KD for injection |
| 259326 | pAJH42 | sapI destination for PhIT tags |
| 259327 | pAJH57 | Germline PhiC31 for injection |
| 259328 | pAJH73 | PhIT tag B3RT2 n-term AID::GFP |
| 259329 | pAJH77 | PhIT tag rox C-term Flpon::Skylan-S |
| 259330 | pAJH83 | rox destination vector for PhIT transgene insertions |
| 259332 | pKJK378 | PhIT tag rox c-term mKate2::stop |
| 259333 | pKJK380 | PhIT tag rox n-term mKate |
| 259334 | pKJK381 | PhIT tag rox N-term mKate2::FLPon |
| 259335 | pKRW1 | PhIT tag B3RT2 n-term GFP |
| 259336 | pKRW11 | PhIT tag B3RT2 c-term Skylan-S::stop |
| 259337 | pKRW2 | PhIT tag B3RT2 n-term 7xGFP11 |
| 259338 | pKRW23 | PhIT tag rox c-term Skylan-S::stop |
| 259339 | pKRW31 | PhIT tag rox n-term GFP |
| 259340 | pKRW32 | PhIT tag rox n-term Skylan-S |
| 259341 | pKRW33 | PhIT tag rox c-term GFP::stop |
| 259342 | pKRW5 | PhIT tag B3RT2 n-term Skylan-S |
| 259343 | pKRW8 | PhIT tag B3RT2 AID::GFP::stop |
| 259346 | pSBJ10 | PhIT tag B3RT2 n-term mTagBFP2 |
| 259347 | pSBJ11 | PhIT tag B3RT2 n-term mStayGold2 |
| 259348 | pSBJ12 | PhIT tag B3RT2 n-term mVenusQ69M |
| 259349 | pSBJ13 | PhIT tag B3RT2 n-term rsFusionRed3 |
| 259350 | pSBJ14 | PhIT tag rox n-term mTagBFP2 |
| 259351 | pSBJ15 | PhIT tag rox n-term mStayGold2 |
| 259352 | pSBJ16 | PhIT tag rox n-term mVenusQ69M |
| 259353 | pSBJ17 | PhIT tag rox n-term rsFusionRed3 |
| 259354 | pSBJ19 | PhIT tag rox c-term mTagBFP2::stop |
| 259356 | pSBJ20 | PhIT tag rox c-term mStayGold2::stop |
| 259357 | pSBJ21 | PhIT tag rox c-term mVenusQ69M::stop |
| 259358 | pSBJ22 | PhIT tag rox c-term rsFusionRed3::stop |
| 259359 | pSBJ23 | PhIT tag rox c-term AID::GFP::stop |
| 259360 | pSBJ5 | PhIT tag B3RT2 c-term 7xGFP11::stop |
| 259361 | pSBJ6 | PhIT tag B3RT2 c-term mStayGold::stop |
| 259362 | pSBJ7 | PhIT tag B3RT2 c-term mVenusQ69M::stop |
| 259363 | pSBJ8 | PhIT tag B3RT2 c-term rsFusionRed3::stop |
| 259364 | pSBJ9 | PhIT tag B3RT2 c-term mTagBFP2::stop |
| 259365 | pAJH67 | PhIT tag rox n-term AID::GFP |
| 259366 | pBORM7 | PhIT tag rox n-term GFP::FLPon |
| 259367 | pEZ35 | PhIT tag rox n-term Skylan-S::FLPon |
| 259368 | pKRW14 | PhIT tag B3RT2 n-term GFP::FLPon |
| 259369 | pKRW36 | PhIT tag B3RT2 n-term mKate2::FLPon |
| 259370 | pKRW18 | PhIT tag B3RT2 n-term Skylan-S::FLPon |
| 259371 | pKRW16 | PhIT tag B3RT2 n-term 7xGFP11::FLPon |
| 259372 | pKJK379 | PhIT tag rox c-term FLPon::mKate2 |
| 259373 | pKRW37 | PhIT tag B3RT2 c-term FLPon::GFP |
| 259374 | pKRW43 | PhIT tag B3RT2 c-term FLPon::mKate2 |
| 259375 | pKRW40 | PhIT tag B3RT2 c-term FLPon::Skylan-S |
| 259426 | pAJH156 | PhIT tag B3RT1 c-term mKate2::stop |