We narrowed to 9,466 results for: CAG

1. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-FRT-MCS-WPRE-SV40 - GCH27

   Plasmid

   #230828

   Purpose

   AAV cloning vector for Flpo-dependent expression under the TRE promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV; Cloning vector, flp/frt

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

   Plasmid

   #102245

   Purpose

   Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

   Depositor

   Jacob Corn

   Insert

   dCas9-VPR-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-VPR-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCellFree_KA1_SARS-COV-2_NSP2

   Plasmid

   #169377

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP2
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCellFree_KA1_SARS-COV-2_NSP3

   Plasmid

   #169378

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP3
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCellFree_KA1_SARS-COV-2_ORF9b

   Plasmid

   #169396

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF9b
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCellFree_KA1_SARS-COV-2_NSP9

   Plasmid

   #169383

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP9
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCellFree_KA1_SARS-COV-2_NSP12

   Plasmid

   #169385

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP12
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCellFree_KA1_SARS-COV-2_NSP15

   Plasmid

   #169388

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP15
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCellFree_KA1_SARS-COV-2_NSP5

    Plasmid

    #169380

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP5
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCellFree_KA1_SARS-COV-2_NSP4

    Plasmid

    #169379

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP4 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP4
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    Aug. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCellFree_KA1_SARS-COV-2_NSP8

    Plasmid

    #169382

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP8 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP8
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA1_SARS-COV-2_NSP13

    Plasmid

    #169386

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP13 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP13
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCellFree_KA1_SARS-COV-2_ORF9c

    Plasmid

    #169397

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9c with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF9c
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCellFree_KA1_SARS-COV-2_NSP14

    Plasmid

    #169387

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP14 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP14
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCellFree_KA1_SARS-COV-2_NSP7

    Plasmid

    #169381

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP7 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP7
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. miABE

    Plasmid

    #205412

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ai66(RCRL-tdT) targeting vector

    Plasmid

    #61578

    Purpose

    Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-RSR-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    CAG

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-U6-sgCTG

    Plasmid

    #232723

    Purpose

    SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    SpCas9 gRNA targeting CAG repeats
    

    Expression

    Mammalian

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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