...pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-tTA AAV2...repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This... vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter. Use this...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls...

... 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral vectors...

... Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype ...AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor Looger New serotype pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype...AAV9 Biosensor St-Pierre New viral vector pAAV-CAG-RdLight1 AAV9 Biosensor Tian New viral vector pAAV-hSynapsin1....A184S AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics ...

...pAAV.CMV.PI.EGFP.WPRE.bGH 37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472...AAV1, 2, 5, 8, 9 (20 µL size serotype testing) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing)... Plasmid Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c... 37825  AAVrg (20 µL size serotype testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...serotypes. This collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465...

...to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new...Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype...AAV5 Biosensors Loren Looger New serotype pAAV-CAG-FLEx-iSeroSnFR AAV9 Biosensors Lin Tian New viral...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714... potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details...collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO...

... Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich New viral...Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE AAV5 Biosensor GENIE Project New...

...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter...

...pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G AAV1, AAV2, AAV9 Biosensor Robert Campbell...Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott ... Name Serotype Category Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor...

...sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST...) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...

...AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) ...Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward...

...from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has tested in a variety...

...drive different levels of expression. For example, CAG is a strong promoter that drives very high, ubiquitous...

...Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 ...

...pAAV-CAG-iSeroSnFR-Nlgn CAG iSeroSnFR none Constitutive 9 Tian 128486 pAAV-CAG-FLEx-iSeroSnFR CAG iSeroSnFR...dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, ...none Constitutive 1, 9 Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Looger...dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg*...none Constitutive 1, 9 Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg* Looger...dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent 1, 9, rg*...Constitutive 1, 9, rg* Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG jGCaMP8m none Constitutive 1, 9, rg* Looger...

...Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru...pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP Control Boyden...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 59462 pAAV-CAG-tdTomato CAG tdTomato Control...-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell...Control Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390...Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent ChR2 Gradinaru...

...expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen construct in mouse. Image from ...

...Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s, Cre-dependent Calcium sensor Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG GCaMP8s, Cre-dependent Calcium sensor GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...-WPRE CAG GCaMP8m, Cre-dependent Calcium sensor GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG GCaMP8f...179254 AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m ...mCherry, Cre-dependent Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 50457 pAAV-hSyn-DIO-EGFP...pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 112677 pOTTC1032 - pAAV...

...104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive...pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru...51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP ...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10...GFAP104 mCherry Constitutive 5 Boyden 59462 pAAV-CAG-tdTomato CAG tdTomato Constitutive 1, 2, 5, 8, 9, rg*, ...Constitutive 8 Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB ...tdTomato Flp dependent 1 Jensen 99133 pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru 137131...