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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,185 results for: golden gate

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Showing: 3301 - 3320 of 5185 results

  1. pJF89

    Plasmid
    #253756
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RNR2 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253756-plasmid-map-sequence-id-509520

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  2. pJF90

    Plasmid
    #253757
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    REV1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253757-plasmid-map-sequence-id-509519

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  3. pJF91

    Plasmid
    #253758
    Purpose
    Encodes GFP_AID* and pADH1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    ADH1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253758-plasmid-map-sequence-id-509518

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  4. pSL160

    Plasmid
    #252932
    Purpose
    Cumate sensing in the sender Escherichia coli "Marionette" strain with RBSEc1 for use in e-COSENS
    Depositor
    Caroline Ajo-Franklin
    Article
    Li et al Nat Biotechnol (2026).
    Insert
    menD
    Expression
    Bacterial
    Promoter
    PCymRC
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    252932-plasmid-map-sequence-id-509418

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  5. pSL178

    Plasmid
    #252937
    Purpose
    Arsenite sensing in the sender Escherichia coli "Marionette" strain with sRBSarsR1 for use in e-COSENS
    Depositor
    Caroline Ajo-Franklin
    Article
    Li et al Nat Biotechnol (2026).
    Insert
    arsR, menD
    Expression
    Bacterial
    Promoter
    PJ109, ParsOC2
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    252937-plasmid-map-sequence-id-509417

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  6. pJF86

    Plasmid
    #253753
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RPL18B and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253753-plasmid-map-sequence-id-509401

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  7. pYSD741

    Plasmid
    #253052
    Purpose
    Encodes MFapp8 Sig Pep, Hydrophobin1 (HFB1), MYC-TEV-649-stalk surface display anchor in a yeast expression vector with pTEF2 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Yeast
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253052-plasmid-map-sequence-id-508946

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  8. pYSD704

    Plasmid
    #253013
    Purpose
    Encodes Trichoderma reesei Hydrophobin1 (HFB1) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Bacterial
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253013-plasmid-map-sequence-id-508761

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  11. pJF88

    Plasmid
    #253755
    Purpose
    Encodes GFP_AID* and pSAC6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    SAC6 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253755-plasmid-map-sequence-id-509369

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  12. pYSD344

    Plasmid
    #253044
    Purpose
    Encodes 2xFLAG-tagged Aga2 with Protein A ZZ domain as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2 Protein A
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253044-plasmid-map-sequence-id-508939

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  13. pYSD328

    Plasmid
    #253040
    Purpose
    Encodes the Aga2 signal peptide, anti-ALFA-tag nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-ALFA-tag nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253040-plasmid-map-sequence-id-508937

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  18. pYSD090

    Plasmid
    #252980
    Purpose
    Encodes MYC-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MYC-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252980-plasmid-map-sequence-id-508739

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  19. pYSD089

    Plasmid
    #252979
    Purpose
    Encodes 2xFLAG-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252979-plasmid-map-sequence-id-508738

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  20. pYSD894

    Plasmid
    #253020
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga2 display anchor in a Met15, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253020-plasmid-map-sequence-id-508728

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Showing: 3301 - 3320 of 5185 results