174,134 results

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TALEN-mROSA26 ELD

Plasmid

#60026

Purpose

Expression of a TALEN protein specific for mouse ROSA26 locus.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Insert

TALEN-ROSA26

Use

TALEN

Expression

Mammalian

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-mROSA26 KKR

Plasmid

#60025

Purpose

Expression of a TALEN protein specific for mouse ROSA26 locus.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Insert

TALEN-ROSA26

Use

TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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LentiGuide-hygro

Plasmid

#160090

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Expression

Yeast

Promoter

PGK1

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-p97

Plasmid

#85670

Purpose

Expression of p97 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1

Plasmid

#213628

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSL3617 (pCRISPR(BbsI)_Pse)

Plasmid

#200902

Purpose

Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-EGFP-WPRE (AAV9)

Viral Prep

#51502-AAV9

Purpose

Ready-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Promoter

CAG

Tags

EGFP (Cre-dependent)

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only
GFP-BRD4

Plasmid

#65378

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD4 (BRD4 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

R1232W

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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HP17deltaP8

Plasmid

#235447

Purpose

Helper plasmid for regulated phage transfer

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#20972

Purpose

H2B fused to mCherry for chromatin visualization

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 flag p53

Plasmid

#10838

Depositor

Thomas Roberts

Article

Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

Insert

p53 (TP53 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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