167,039 results

1. pCDNA4.TO-ORF37-2xCSTREP

   Plasmid

   #136198

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF37
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-hygro-eGFP-HEC1

   Plasmid

   #114027

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JN202: pMVP/SB/Blast-DEST

   Plasmid

   #121864

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEM mouse dbx-1 [TJ#11]

   Plasmid

   #16269

   Depositor

   Thomas Jessell

   Article

   Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

   Insert

   dbx1 3'UTR (Dbx1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

5. PB_TRE_AP4-PDGFR_EF1a_eBFP2

   Plasmid

   #186306

   Purpose

   Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP4-PDGFR
   eBFP2
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTurquoise2-N1

   Plasmid

   #54843

   Purpose

   Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

   Depositor

   Michael Davidson, Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. TFORF0120

   Plasmid

   #142539

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ELMSAN1 (MIDEAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EBFP2-Cx30

   Plasmid

   #69022

   Purpose

   Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx30 (GJB6 Human)
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

    Plasmid

    #139308

    Purpose

    N-terminal half of split PI-PLC targeted to plasma membrane

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. negAKARet-cyto

    Plasmid

    #114733

    Purpose

    Negative control of AKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of AKARet-cyto
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1346

    Plasmid

    #119274

    Purpose

    knockdown folA in K. pneumoniae

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (K. pneumoniae)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dronpa-FHA1

    Plasmid

    #87711

    Purpose

    Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Dronpa-FHA1
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB_TRE_sg83-PDGFR_EF1a_iRFP670

    Plasmid

    #186311

    Purpose

    Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg83-PDGFR
    iRFP670
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid

    #186312

    Purpose

    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg88-PDGFR
    eBFP2
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid

    #186307

    Purpose

    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P9-PDGFR
    mPlum
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid

    #186308

    Purpose

    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP10-PDGFR
    mOrange
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid

    #186305

    Purpose

    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P3-PDGFR
    iRFP670
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUPERretro-Sirt7 shRNA2

    Plasmid

    #53145

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pSUPERretro-Sirt7 shRNA1

    Plasmid

    #53144

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only