162,925 results

1. Flag-FOXI1

   Plasmid

   #153126

   Purpose

   Expression of Flag tagged human FOXI1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXI1 (FOXI1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GRK2-CAAX

   Plasmid

   #166224

   Purpose

   GRK2-CAAX mammalian expression plasmid

   Depositor

   Robert Lefkowitz

   Article

   Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

   Insert

   bovine GRK2-CAAX (GRK2 Bovine)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   adding CAAX sequence at C terminal

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mARGAna (cassette 1)

   Plasmid

   #191341

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-10X-CS1-3'-puro

   Plasmid

   #212011

   Purpose

   Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

   Depositor

   Danwei Huangfu

   Insert

   gRNA scaffold
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-V5 [IPI-SV5-Pk1] (Recombinant Antibody)

   Antibody

   #218107-rAb

   Purpose

   Anti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Randall et al J Gen Virol. 1987 Nov;68 ( Pt 11):2769-8

   Recommended Applications

   Western Blot

   Reactivity

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   May 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

6. prhaBAD-pA-Hia5

   Plasmid

   #222303

   Purpose

   Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   pA-Hia5
   

   Use

   Tags

   6His and protein A

   Expression

   Bacterial

   Mutation

   codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTetBl-CLIP-ALMS1

   Plasmid

   #136877

   Purpose

   Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-ALMS1 (ALMS1 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR26-Tango

   Plasmid

   #66348

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR26 (GPR26 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSMrnaSeph

    Plasmid

    #67507

    Purpose

    Express the tRNAMet scaffold with the sephadex aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNAMet with sephadex aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

    Plasmid

    #55222

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Matr3 delRRM2

    Plasmid

    #32882

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 497-566

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-SBP-TEV

    Plasmid

    #36943

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHistag-SBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. FLAG-Matr3 delRRM1

    Plasmid

    #32881

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 399-468

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pTNT-B18R-6His

    Plasmid

    #58979

    Purpose

    expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Sp6

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Use

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 39R861+

    Bacterial Strain

    #119737

    Purpose

    Six stable plasmids can be used as size standards

    Depositor

    Ruth Hall

    Article

    Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

    Bacterial Resistance

    None

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pET28_6xHis-PARP1delBRCT

    Plasmid

    #173938

    Purpose

    Bacterial expression of PARP1 lacking BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delBRCT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 374-520; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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