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Addgene

pLenti.PGK.LifeAct-Ruby.W
(Plasmid #51009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti.PGK:EGFP
  • Backbone manufacturer
    L. Naldini
  • Total vector size (bp) 7462
  • Modifications to backbone
    Digested pmRFPruby-N1-Lifeact-EcoRI-BHI with Nhe1/Not1 (T4 DNAP) and ligated into RRLsin.cPPT.hPGK.eGFP.Wpre (Naldini) BamH1/Sal1/T4 DNAP/CIP vector.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LifeAct-mRFPruby
  • Insert Size (bp)
    774
  • Promoter human PGK
  • Tag / Fusion Protein
    • LifeAct (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site Sal1 (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti.PGK.LifeAct-Ruby.W was a gift from Rusty Lansford (Addgene plasmid # 51009 ; http://n2t.net/addgene:51009 ; RRID:Addgene_51009)
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