178,448 results
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Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
ExpressionBacterialMutationE32KAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-puro-v2
Plasmid#127458PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSyn.iGluSnFr.WPRE.SV40 (AAV9)
Viral prep#98929-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.hSyn.iGluSnFr.WPRE.SV40 (#98929). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFr.WPRE.SV40 plasmid DNA. Synapsin-driven, iGluSnFr glutamate sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-EGFP
Plasmid#89875PurposeEGFP reporter vector of iTango system for AAV productionDepositorInserttetO-EGFP
UseAAVExpressionMammalianPromotertetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorPublicationInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas serviceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-Blue FP
Plasmid#52115Purposea bicistronic IRES-Blue FP containing retroviral vectorDepositorTypeEmpty backboneUseRetroviralTagsIRES-Blue FPExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human Lentiviral sgRNA Library - Nuclear
Pooled library#51047PurposeSub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorPublicationInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
ExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas serviceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-mCitrine-Sox2
Plasmid#23242DepositorInsertmCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
UseLentiviralAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by