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pCS2-nCas9n-nanos 3’UTR
(Plasmid #62542)


Item Catalog # Description Quantity Price (USD)
Plasmid 62542 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8272
  • Total vector size (bp) 8910
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    nanos 3' UTR
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Mutation
    3' UTR only
  • Entrez Gene
    nanos3 (a.k.a. cb725, nanos, nanos1, nos1)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ATCaccggtAGCGGACATTGATGCTCC
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

vector backbone was Addgene Plasmid #47929 from Wenbiao Chen

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ; ; RRID:Addgene_62542)
  • For your References section:

    CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, Khokha MK, Giraldez AJ. Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543. 10.1038/nmeth.3543 PubMed 26322839