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CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo.
Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, Khokha MK, Giraldez AJ
Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62542pCS2-nCas9n-nanos 3’UTRexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish
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Antibodies from Article