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Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain.
Lee D, Creed M, Jung K, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB
Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89869pCMV-bArrestin2-TevC-P2A-TdTomatoexpresses iTango bArrestin2-TevC component and TdTomato bicistronically
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89870pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16expresses DRD2-iTango component in mammalian cells
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89871pTRE-EGFPEGFP reporter vector for iTango system
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89872pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16expresses CB1R-iTango component in mammalian cells
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89873pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpAexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
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89874pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpAexpresses DRD2-iTango component in mammalian cells for AAV production
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89875pAAV-TRE-EGFPEGFP reporter vector of iTango system for AAV production
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89876pAAV-TRE-FLEX-eNpHR-EYFPTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter
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89877pCMV-NES-CRY2PHR-TevCexpresses NES-CRY2PHR-TevC of BLITz system
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89878pCMV-TM-CIBN-BLITz1-TetR-VP16expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system
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