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165,839 results

Showing: 3361 - 3380 of 165839 results

  3. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T3 promoter
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  4. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (AAV9)

    Viral Prep
    #100061-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (#100061). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) plasmid DNA. EFIa-driven, Cre-dependent, C1V1 (t/t) fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.eB)

    Viral Prep
    #59462-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  12. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179390-plasmid-map-sequence-id-451660

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  16. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

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  17. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV9)

    Viral Prep
    #35509-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3361 - 3380 of 165839 results