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Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.
Tiller T, Meffre E, Yurasov S, Tsuiji M, Nussenzweig MC, Wardemann H
J Immunol Methods. 2008 Jan 1;329(1-2):112-24. Epub 2007 Oct 31.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80795AbVec2.0-IGHG1Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype
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80796AbVec1.1-IGKCExpression of immunoglobulin light chains in mammalian cells, human kappa constant region
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99575AbVec1.1-IGLC2-XhoIExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region
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Recombinant Antibodies from Article