Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.
Tiller T, Meffre E, Yurasov S, Tsuiji M, Nussenzweig MC, Wardemann H
J Immunol Methods. 2008 Jan 1;329(1-2):112-24. Epub 2007 Oct 31. PubMed Article
Tiller T, Meffre E, Yurasov S, Tsuiji M, Nussenzweig MC, Wardemann H
J Immunol Methods. 2008 Jan 1;329(1-2):112-24. Epub 2007 Oct 31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80795 | AbVec2.0-IGHG1 | Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype |
| 80796 | AbVec1.1-IGKC | Expression of immunoglobulin light chains in mammalian cells, human kappa constant region |
| 99575 | AbVec1.1-IGLC2-XhoI | Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region |