Purpose(Empty Backbone) Expression of immunoglobulin light chains in mammalian cells, human kappa constant region
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80796||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5020
Vector typeMammalian Expression
- Promoter hCMV IE1 gene promoter
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GCTTCGTTAGAACGCGGCTAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Annotated sequence available from:
accession number LT615369
Also used in Tiller et al., J Immunol Methods. 2009 Oct 31;350(1-2):183-93
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AbVec1.1-IGKC was a gift from Hedda Wardemann (Addgene plasmid # 80796 ; http://n2t.net/addgene:80796 ; RRID:Addgene_80796)
For your References section:Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning. Tiller T, Meffre E, Yurasov S, Tsuiji M, Nussenzweig MC, Wardemann H. J Immunol Methods. 2008 Jan 1;329(1-2):112-24. Epub 2007 Oct 31. 10.1016/j.jim.2007.09.017 PubMed 17996249