Purpose(Empty Backbone) Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80797||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5018
Vector typeMammalian Expression
- Promoter hCMV IE1 gene promoter
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GCTTCGTTAGAACGCGGCTAC (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Annotated sequence available from:
accession number LT615370
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AbVec2.1-IGLC2-MscI was a gift from Hedda Wardemann (Addgene plasmid # 80797 ; http://n2t.net/addgene:80797 ; RRID:Addgene_80797)
For your References section:Cloning and expression of murine Ig genes from single B cells. Tiller T, Busse CE, Wardemann H. J Immunol Methods. 2009 Oct 31;350(1-2):183-93. doi: 10.1016/j.jim.2009.08.009. Epub 2009 Aug 27. 10.1016/j.jim.2009.08.009 PubMed 19716372