165,820 results

1. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGWB-nLUC

   Plasmid

   #174050

   Purpose

   Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   N-terminal luciferase

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)

   Viral Prep

   #105530-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pD4sE_SC12

   Plasmid

   #219721

   Purpose

   DV4 stable E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   DV4 sE SC12
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…

   Promoter

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pD1sE_SC12

   Plasmid

   #219718

   Purpose

   DV1 E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   DV1 sE SC12
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…

   Promoter

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pttq18

   Plasmid

   #69122

   Purpose

   regulated expression of genes in Escherichia coli

   Depositor

   Michael Stark

   Article

   Stark et al Gene. 1987;51(2-3):255-67.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

   Viral Prep

   #50942-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Tobias Rose, Tobias Bonhoeffer, Mark Huebener

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Promoter

   Syn

   Tags

   mRuby2

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pHR- flag-Lag16-Ecad

   Plasmid

   #205197

   Purpose

   This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM Ecad and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD2sE_SC12

    Plasmid

    #219719

    Purpose

    DV2 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV2 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHSE901

    Plasmid

    #91708

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcsII-pb5-flag

    Plasmid

    #86769

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 5 (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MORF library without GFP and mCherry controls

    Pooled Library

    #192821

    Purpose

    Barcoded transcription factor ORF library for high throughput screening

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

    Plasmid

    #216694

    Purpose

    Synchronize trafficking of GPI-AP from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    SBP-mApple-FIREtag-GPI
    

    Use

    Tags

    FIREtag, IL2 signal sequence, Streptavidin Bindin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG1344 EGFP-FIREtag

    Plasmid

    #205165

    Purpose

    Expresses EGFP-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    EGFP-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge