163,882 results

1. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

2. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

   Plasmid

   #104563

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1 (D832A)
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_GEM4-Halo-FRB

   Plasmid

   #197057

   Purpose

   Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHes1-DN-GFP (CC#110)

   Plasmid

   #15134

   Depositor

   Connie Cepko

   Article

   Furukawa et al Neuron. 2000 May . 26(2):383-94.

   Insert

   Hes1 (Hes1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E43A, K44A, R47A

   Promoter

   Available since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHes1-DsRed

   Plasmid

   #13767

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Hes1 promoter
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCM’

   Plasmid

   #162709

   Purpose

   pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   p15A origin replaced with high-copy colE1 origin

   Promoter

   PLtet0-1 promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSP33

   Plasmid

   #199375

   Purpose

   Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Promoter

   Available since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV2/rh10

    Plasmid

    #112866

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap-rh10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV3flag8HOIP

    Plasmid

    #50015

    Purpose

    Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    ring finger protein 31 (RNF31 Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Rab5C-S35N

    Plasmid

    #110503

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pHS15 (hANGPTL3-Strep)

    Plasmid

    #109112

    Purpose

    Expresses StrepII-tagged human ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (ANGPTL3 Human)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197064

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_COCVmut_G

    Plasmid

    #207262

    Purpose

    Mammalian expression of a mutant Cocal virus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    COCVmut_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47A/Y209A/R354A; mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge