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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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252,492 results

Showing: 3401 - 3420 of 252492 results

  1. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  10. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74224-plasmid-map-sequence-id-238509

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  11. pAAV-hsyn-GRAB_DA2h (AAV9)

    Viral Prep
    #140554-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

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  13. pKL2299A

    Plasmid
    #222105
    Purpose
    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.
    Depositor
    Kan Wang
    Article
    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.
    Insert
    virA
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    virA promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222105-plasmid-map-sequence-id-442317

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  17. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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Showing: 3401 - 3420 of 252492 results