DHB-mVenus
(Plasmid #136461)

• Purpose: Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2
• Depositing Labs: Tobias Meyer, Sabrina Spencer
• Publication: Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10.1016/j.cell.2013.08.062. Epub 2013 Sep 26.

Backbone

• Vector backbone: CSII-EF-MCS
• Backbone size w/o insert (bp): 9136
• Total vector size (bp): 10159
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DNA Helicase B
• Alt name: DHB
• Species: H. sapiens (human)
• Insert Size (bp): 1023
• Entrez Gene: HELB (a.k.a. DHB, hDHB)
• Tag / Fusion Protein:
  • mVenus (C terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry