PurposeFluorescent fusion protein for tracking Akt signaling
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67759||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneAddgene Plasmid: 17452
- Backbone size w/o insert (bp) 8679
- Total vector size (bp) 11358
Modifications to backboneReplaced CMV promoter with EF1a
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameForkhead box protein O1
SpeciesM. musculus (mouse)
Insert Size (bp)2679
MutationChanged Serine 209 to Alanine, changed Histidine 212 to Arginine, changed Serine 215 to Alanine
Entrez GeneFoxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)
- Promoter EF1a
/ Fusion Protein
- Clover (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Pac1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: Addgene's quality control sequencing finds four additional amino acid residue substitutions in the murine FoxO1 insert: Q275R, N515Y, S574N, and L619P. The depositing laboratory has tested this construct biochemically and determined that these residue changes do not detrimentally affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-FoxO1-Clover was a gift from Peter Rotwein (Addgene plasmid # 67759 ; http://n2t.net/addgene:67759 ; RRID:Addgene_67759)
For your References section:Akt signaling dynamics in individual cells. Gross SM, Rotwein P. J Cell Sci. 2015 Jun 3. pii: jcs.168773. 10.1242/jcs.168773 PubMed 26040286