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pLenti-FoxO1-Clover
(Plasmid #67759)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67759 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Addgene Plasmid: 17452
  • Backbone size w/o insert (bp) 8679
  • Total vector size (bp) 11358
  • Modifications to backbone
    Replaced CMV promoter with EF1a
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fox01
  • Alt name
    Forkhead box protein O1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2679
  • Mutation
    Changed Serine 209 to Alanine, changed Histidine 212 to Arginine, changed Serine 215 to Alanine
  • Entrez Gene
    Foxo1 (a.k.a. AI876417, Afxh, FKHR, Fkhr1, Foxo1a)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Clover (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pac1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    FOXO1: Addgene Plasmid 34678 Clover: Addgene Plasmid 40259
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Note: Addgene's quality control sequencing finds four additional amino acid residue substitutions in the murine FoxO1 insert: Q275R, N515Y, S574N, and L619P. The depositing laboratory has tested this construct biochemically and determined that these residue changes do not detrimentally affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-FoxO1-Clover was a gift from Peter Rotwein (Addgene plasmid # 67759 ; http://n2t.net/addgene:67759 ; RRID:Addgene_67759)
  • For your References section:

    Akt signaling dynamics in individual cells. Gross SM, Rotwein P. J Cell Sci. 2015 Jun 3. pii: jcs.168773. 10.1242/jcs.168773 PubMed 26040286