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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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171,627 results

Showing: 3441 - 3460 of 171627 results

  1. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    AAV
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  6. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available Since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

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  8. pN1-OPA1(123Nt)-Halo

    Plasmid
    #129345
    Purpose
    Halo Tag, Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    Halo (Promega)
    Expression
    Mammalian
    Available Since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129345-plasmid-map-sequence-id-260109

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  12. pcDNA-Pom121 mApple

    Plasmid
    #187674
    Purpose
    In vivo visualization of NPC protein Pom121
    Depositor
    Mary Beckerle
    Insert
    Pom121 (Pom121 Mouse)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    187674-plasmid-map-sequence-id-371227

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  15. DRD2-DuET

    Plasmid
    #213228
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD2 (DRD2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213228-plasmid-map-sequence-id-430789

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  17. pSBtet-Bla/PAG1-GFP

    Plasmid
    #198220
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198220-plasmid-map-sequence-id-389402

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  20. TFORF2514

    Plasmid
    #144124
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PDCD2 (PDCD2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144124-plasmid-map-sequence-id-396186

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Showing: 3441 - 3460 of 171627 results