163,905 results

1. pTH2

   Plasmid

   #84452

   Purpose

   shuttle plasmid for sarA P1-CFP expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   CFP Cerulean
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-MBP_G4G6 in pRSF

   Plasmid

   #188454

   Purpose

   Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   G4G6 (GSN Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_BRAF_p.G469V

   Plasmid

   #82833

   Purpose

   Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G469V

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT/Caggs-NRASV12

   Plasmid

   #20205

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   NRas (NRAS Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACS145

   Plasmid

   #204600

   Purpose

   Histone H3.3K36M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.3 K36M (H3-3A Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.3 K36M - V5

   Expression

   Mammalian

   Mutation

   histone H3.3 K36M

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMVtight Blast DEST (w762-1)

   Plasmid

   #26434

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Lentiviral; Tet-on advanced destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2079

   Plasmid

   #141911

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV1)

   Viral Prep

   #100054-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CAG

   Tags

   mCherry

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pLib6.4

    Plasmid

    #133783

    Purpose

    Library backbone

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIPZ shSTING-Mmu

    Plasmid

    #127697

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse STING gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shSTING Mmu (Sting1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF HaloTag-SYT1

    Plasmid

    #197280

    Purpose

    Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-Kinesin11-N-18

    Plasmid

    #54137

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. p3E-TurboID

    Plasmid

    #166570

    Purpose

    Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD/HisB-iRFP702

    Plasmid

    #45455

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP702
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

    Promoter

    araBAD

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. psigma2-EGFP

    Plasmid

    #53610

    Purpose

    Expresses sigma2 of adaptor protein of AP2

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma2 (Ap2s1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Zeo-mEGFP

    Plasmid

    #214145

    Purpose

    Expresses the monomeric EGFP in eukaryotic cells

    Depositor

    Bertrand Collet

    Article

    Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A207K L221K F223R

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBA1015

    Plasmid

    #189589

    Purpose

    T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    T7gp1.7 Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of T7gp1.7

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIPZ shcGAS-Mmu

    Plasmid

    #127698

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse cGAS gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shcGAS Mmu (Cgas Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge