163,977 results

1. pDGE333

   Plasmid

   #153242

   Purpose

   M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lentiviral-S-tdTomato

   Plasmid

   #112579

   Purpose

   Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

   Depositor

   Jeffrey Rothstein

   Article

   Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

   Insert

   NLS-tdTomato-NES
   

   Use

   Lentiviral

   Tags

   nuclear export sequence and nuclear localization …

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTR

   Plasmid

   #63143

   Purpose

   PCR template to construct polycistronic tRNA-gRNA

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   gRNA-tRNA fusion
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. C3AR1-DuET

   Plasmid

   #213191

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   C3AR1 (C3AR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-Jaws-KGC-GFP-ER2

   Plasmid

   #65015

   Purpose

   AAV-mediated expression of Jaws under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ADRA2C-Tango

   Plasmid

   #66218

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA2C (ADRA2C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDGE335

   Plasmid

   #153244

   Purpose

   M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OXTR-Tango

   Plasmid

   #66467

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   OXTR (OXTR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. 4xMS2-hTR

   Plasmid

   #154940

   Purpose

   4xMS2 stem-loop-hTR-500 bp genomic sequence

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   4xMS2 stem-loop-hTR-500 bp genomic sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKJ0463

    Plasmid

    #228715

    Purpose

    Bacterial expression of epT7 RNAP; for protein production and purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    Sumo_epT7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T3M/G47A/E643G

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Luciferase-pcw107-V5

    Plasmid

    #64649

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    PGK

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223_EGFR_p.ELREA_746del

    Plasmid

    #82911

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Deletion of residues 746-750 (ELREA)

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sigma 70 promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

    Plasmid

    #171437

    Purpose

    Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    c-Fos-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mito-CanlonicSF/pCMV-myc-mito

    Plasmid

    #224465

    Purpose

    To explore mitochondrial lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Rauseo et al bioRxiv 2024.07.19.604361

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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