165,839 results

1. pCE059-SiT-Cas12a-[Activ]

   Plasmid

   #128136

   Purpose

   Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Activ]-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-HA-eLACCO2.1-NGR

   Plasmid

   #208023

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Sec23A

   Plasmid

   #166893

   Purpose

   mammalian expression of Sec23A tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Sec23A

   Plasmid

   #66609

   Purpose

   GFP fusion of Sec23A

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

   Insert

   Sec23A (SEC23A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT281

   Plasmid

   #122611

   Purpose

   express evoAPOBEC1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoAPOBEC1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E4K H109N H122L D124N R154H A165S P201S F205S

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Flag hHMGB1

   Plasmid

   #31609

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-mScarlet (AAV1)

   Viral Prep

   #131001-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Promoter

   Syn

   Tags

   mScarlet

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-EF1a-fDIO-Cre (AAV9)

   Viral Prep

   #121675-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Alexander Nectow, Esteban Engel

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Promoter

   EF1a

   Tags

   None

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-NUP98

    Plasmid

    #229593

    Purpose

    Expresses EGFP-NUP98 fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep

    #50942-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Mark Huebener, Tobias Bonhoeffer, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Promoter

    Syn

    Tags

    mRuby2

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416GPD-ccdB

    Plasmid

    #14148

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. RNA-binding protein pooled CRISPR knockout library

    Pooled Library

    #141438

    Purpose

    CRISPR knockout library targets RBPs with 10 sgRNAs/gene

    Depositor

    Eugene Yeo

    Article

    Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pBZS-M.CviPII (NDel15)

    Plasmid

    #198356

    Purpose

    Express M.CviPII (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge