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182,076 results

Showing: 3581 - 3600 of 182076 results

  1. pCMV-dCas13-M3nls

    Plasmid
    #155366
    Purpose
    Targeted m6A RNA methylation in mammalian cells
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155366-plasmid-map-sequence-id-305808

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  2. pcDNA3.1-SARS-Spike

    Plasmid
    #145031
    Purpose
    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells
    Depositor
    Fang Li
    Publication
    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV Spike (S Synthetic)
    Tags
    C9 and CD5 signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 16, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145031-plasmid-map-sequence-id-292651

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  10. HIP-flash (HOP-flash mutant)

    Plasmid
    #83466
    Purpose
    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Publication
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83466-plasmid-map-sequence-id-159299

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  14. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B (H2BC11 Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    55243-plasmid-map-sequence-id-159436

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  16. mCherry-CD36-C-10

    Plasmid
    #55011
    Purpose
    Localization: Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD36 (CD36 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55011-plasmid-map-sequence-id-313445

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  18. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47754-plasmid-map-sequence-id-68222

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  20. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Publication
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    37184-plasmid-map-sequence-id-46727

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Showing: 3581 - 3600 of 182076 results