165,839 results

1. C321.ΔA

   Bacterial Strain

   #48998

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Ampicillin

   Species

   Escherichia coli

   Available Since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-LC3 (human)

   Plasmid

   #24920

   Purpose

   Mammalian expression of LC3 fused to EGFP

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Con/Foff 2.0-EYFP (AAV8)

   Viral Prep

   #137162-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-EYFP (#137162). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-EYFP plasmid DNA. EF1a-driven expression of EYFP in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pLJC5-Tmem192-2xFlag

   Plasmid

   #102929

   Purpose

   Express a lysosomal Tag

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330S-3

   Plasmid

   #58779

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-FLuc

   Plasmid

   #170575

   Purpose

   Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   Luc
   

   Use

   Luciferase and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC0940 (circRNA entry vector)

   Plasmid

   #189967

   Purpose

   Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. v5 Cas9-eVLP

   Plasmid

   #228468

   Purpose

   Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

   Depositor

   David Liu

   Article

   Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

   Insert

   MMLVgag(C507V)–Cas9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   MMLVgag(C507V)

   Promoter

   CMV

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. YIp204-PADH1-atTIR1-9myc

    Plasmid

    #99532

    Purpose

    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    9xMyc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

    Plasmid

    #136904

    Purpose

    Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    mScarletGo-p2a-Blas
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available Since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pSH-CAG-AtAFB2.F74A-IRES-puro

    Plasmid

    #216245

    Purpose

    Express AtAFB2.F74A mutant without tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV Retrograde)

    Viral Prep

    #105558-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    None

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-CaMKII-WHaloCaMP1a-EGFP

    Plasmid

    #205309

    Purpose

    Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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