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Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway.
Kim NG, Gumbiner BM
J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
83466HIP-flash (HOP-flash mutant)Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene
83467HOP-flashTEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
83468Tet-myr-GFPLentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells
83469Tet-CA-Src-GFPLentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells
83470Tet-myr-CSK-GFPLentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells
83471Tet-myr-CSK K222R-GFPLentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cells
83472Tet-FRNK-GFPLentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells

Antibodies from Article