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Mouse genome engineering using designer nucleases.
Hermann M, Cermak T, Voytas DF, Pelczar P
J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
37184pCAG-T7-TALEN(Sangamo)-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
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40131pCAG-T7-TALEN(Sangamo)-FokI-KKR-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
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40132pCAG-T7-TALEN(Sangamo)-FokI-ELD-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
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