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(Plasmid #40132)


Item Catalog # Description Quantity Price (USD)
Plasmid 40132 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 4814
  • Vector type
    Mammalian Expression
  • Promoter CAG
  • Tag / Fusion Protein
    • FokI nuclease (ELD) (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The TALEN destination cassette is derived from pTAL4, which is included in the Voytas Lab Golden Gate TALEN Kit. The heterodimeric FokI nuclease, including Q486E, I499L and N496D mutations as well as "Sharkey" mutations S418P and K441E, was derived from Plasmid 37198: pCMV-RosaL6 ELD mutations provided by the Gersbach lab.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Pelczar TALEN Add-On Plasmids please refer to:

Please note that plasmid #40132 is only functional in combination with plasmid #40131.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination was a gift from Pawel Pelczar (Addgene plasmid # 40132 ; ; RRID:Addgene_40132)
  • For your References section:

    Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757